Protein Purification Flow Charts

R.K. Scopes1

1 La Trobe University, Bundoora
Publication Name:  Current Protocols in Protein Science
Unit Number:  Unit 1.3
DOI:  10.1002/0471140864.ps0103s00
Online Posting Date:  May, 2001
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Abstract

Protein purification flow charts are presented to give a broad outline of the methods used for different types of proteins. They cannot give any detail, as the process appropriate for each protein will have its own variations at each stage. In most cases, the first stage is to obtain a solution containing the desired protein, after which it can be fractionated by various separation techniques. This unit provides charts tailored for purification of soluble and insoluble recombinant and nonā€recombinant proteins, as well as membraneā€associated proteins.

     
 
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Table of Contents

  • Soluble Recombinant Proteins
  • Insoluble Recombinant Proteins
  • Soluble Nonrecombinant Proteins
  • Membrane‐Associated and Insoluble Nonrecombinant Proteins
  • Figures
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   Walter, H. and Johansson, G. (eds.) 1994. Aqueous two‐phase systems. Methods Enzymol. 228:1‐725.
   Zeilstra‐Ryalls, J., Fayet, O., and Georgopoulos, C. 1991. The universally‐conserved GroE (Hsp60) chaperonins. Annu. Rev. Microbiol. 45:301‐325.
Key References
   Deutscher, M.P.(ed.) 1990. Guide to protein purification. Methods Enzymol. 182:1‐894.
  Extensive collection of purification methods, with some general protocols and examples.
   Janson, J.‐C. and Ryden, L.G. 1989. Protein Purification: Principles, High Resolution Methods, and Applications. VCH Publishers, New York.
  A useful collection of methods and examples.
   Kennedy, J.F. and Cabral, J.M. (eds.) 1993. Recovery Processes for Biological Materials. John Wiley & Sons, New York.
  A useful introduction to the problems of large‐scale methods.
   Kenny, A. and Fowell, S. (eds.) 1992. Practical protein chromatography. Methods Mol. Biol. 11:1‐327.
  Extensive descriptions of affinity chromatographic techniques with protocols and recipes.
   Scopes, R.K. 1993. Protein Purification, Principles and Practice, 3rd ed. Springer‐Verlag, New York and Heidelberg.
  General principles of all the main techniques used in purifying proteins. A useful laboratory handbook; does not include recipes or procedures for specific proteins.
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