Computational Large‐Scale Mapping of Protein‐Protein Interactions Using Structural Complexes

Benjamin Shoemaker1, Stefan Wuchty1, Anna R. Panchenko1

1 National Institutes of Health, Bethesda, Maryland
Publication Name:  Current Protocols in Protein Science
Unit Number:  Unit 3.9
DOI:  10.1002/0471140864.ps0309s73
Online Posting Date:  September, 2013
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Although the identification of protein interactions by high‐throughput methods progresses at a fast pace, “interactome” datasets still suffer from high rates of false positives and low coverage. To map the interactome of any organism, this unit presents a computational framework to predict protein‐protein or gene‐gene interactions utilizing experimentally determined evidence of structural complexes, atomic details of binding interfaces and evolutionary conservation. Curr. Protoc. Protein Sci. 73:3.9.1‐3.9.9. © 2013 by John Wiley & Sons, Inc.

Keywords: protein interaction; structural complex; binding interface; IBIS; Inferred Biomolecular Interactions Server

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Table of Contents

  • Introduction
  • Basic Protocol 1: Computational Prediction of Protein Interactions on the Organism Scale Using IBIS
  • Commentary
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

Literature Cited
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