Production of Recombinant Proteins in Escherichia coli

Edward R. LaVallie1

1 Genetics Institute, Inc., Cambridge
Publication Name:  Current Protocols in Protein Science
Unit Number:  Unit 5.1
DOI:  10.1002/0471140864.ps0501s00
Online Posting Date:  May, 2001
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Abstract

E. coli is the expression system of choice and a substantial body of literature has accumulated on the successful expression of foreign genes in this host. Several problems with protein expression in E. coli have been encountered, and many have been ultimately solved. This unit describes methods that have been developed for production of recombinant proteins in E. coli and potential pitfalls that may be encountered.

     
 
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Table of Contents

  • General Strategies for Gene Expression in E. Coli
  • Specific Expression Strategies
  • Summary
  • Literature Cited
  • Tables
     
 
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Materials

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Literature Cited

Literature Cited
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