Overview of the Baculovirus Expression System

Cheryl Isaac Murphy1, Helen Piwnica‐Worms2

1 Cambridge Biotech Corporation, Worcester, 2 Washington University School of Medicine, St. Louis
Publication Name:  Current Protocols in Protein Science
Unit Number:  Unit 5.4
DOI:  10.1002/0471140864.ps0504s00
Online Posting Date:  May, 2001
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Abstract

Baculoviruses have emerged as a popular system for overproducing recombinant proteins in eukaryotic cells. This overview unit describes the baculovirus life cycle and expression system, and also provides information on vectors and protocols for using the baculovirus expression system.

     
 
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Table of Contents

  • Baculovirus Life Cycle
  • Baculovirus Expression System
  • Post‐Translational Modification of Proteins in Insect Cells
  • Figures
     
 
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Materials

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Figures

Videos

Literature Cited

Literature Cited
   Carbonell, L.F., Klowden, M.J., and Miller, L.K. 1985. Baculovirus‐mediated expression of bacterial genes in dipteran and mammalian cells. J. Virol. 56:153‐160.
   Doerfler, W. and Bohm, P. 1986. The Molecular Biology of Baculoviruses. Springer‐Verlag, New York.
   Hellers, M., Gunne, H., and Steiner, H. 1991. Expression and post‐translational processing of preprocecropin‐A using a baculovirus vector. Eur. J. Biochem. 199:435‐439.
   Jarvis, D.L. and Summers, M.D. 1992. Baculovirus expression vectors. In Recombinant DNA Vaccines: Rationale and Strategies (R.E. Isaacson, ed.) pp.265‐291. Marcel Dekker, New York.
   Kitts, P.A. and Possee, R.D. 1993. A method for producing recombinant baculovirus expression vectors at high frequency. BioTechniques 14:810‐817.
   Kitts, P.A., Ayres, M.D., and Possee, R.D. 1990. Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucl. Acids Res. 18:5667‐5672.
   Luckow, V.A. 1991. Cloning and expression of heterologous genes in insect cells with baculovirus vectors. In Recombinant DNA Technology and Applications (A. Prokop, R.K. Bajpai, and C. Ho, eds.) pp.97‐152. McGraw‐Hill, New York.
   Luckow, V.A. and Summers, M.D. 1988. Trends in the development of baculoviral expression vectors. Bio/Technology 6:47‐55.
   Miller, L.K. 1988. Baculoviruses as gene expression vectors. Annu. Rev. Microbiol. 42:177‐199.
   Miller, D.W., Safer, P., and Miller, L.K. 1986. An insect baculovirus host vector for high‐level expression of foreign genes. In Genetic Engineering, Vol. 8 (J.K. Setlow and A. Hollaender eds.) pp.277‐298. Plenum, New York.
   O'Reilly, D.R., Miller, L.K., and Luckow, V.A. 1992. Baculovirus Expression Vectors. W.H. Freeman and Company, New York.
   Piwnica‐Worms, H., Williams, N.G., Cheng, S.H., and Roberts, T.M. 1990. Regulation of pp60c‐src and its association with polyoma virus middle T antigen in insect cells. J. Virol. 64:61‐68.
   Summers, M.D. and Smith, G.E. 1987. A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experiment Station Bulletin No. 1555. College Station, Texas.
Key Reference
   O'Reilly et al. 1992. See above.
  A guide assembled to aid researchers using the baculoviral expression system, containing detailed protocols for using this system effectively.
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