Overview of Protein Expression in Saccharomyces cerevisiae

Robert L. Strausberg1, Susan L. Strausberg2

1 National Institutes of Health, Bethesda, 2 University of Maryland, Rockville
Publication Name:  Current Protocols in Protein Science
Unit Number:  Unit 5.6
DOI:  10.1002/0471140864.ps0506s02
Online Posting Date:  May, 2001
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This overview presents vectors and host strains that are available to direct gene expression in S. cerevisiae, including information on promoters, vector maintenance and copy number, transcription terminators, and selectable markers. Challenges to the expression of foreign proteins are also covered, including attainment of desired production yield, production of protein with appropriate postā€translational modifications, conformation and function, and secretion to the extracellular medium.

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Table of Contents

  • Vectors and Host Strains
  • Challenges to the Expression of Foreign Proteins
  • Time Frame and Future Prospects
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

Literature Cited
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