Choice of Cellular Protein Expression System

David Gray1, Shyam Subramanian2

1 Chiron Corporation, Emeryville, California, 2 Merck Research Laboratories, West Point, Pennsylvania
Publication Name:  Current Protocols in Protein Science
Unit Number:  Unit 5.16
DOI:  10.1002/0471140864.ps0516s20
Online Posting Date:  May, 2001
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Recombinant protein expression has become a standard laboratory tool, and a wide variety of systems and techniques are now in use. Because there are so many systems to choose from, the investigator has to be careful to use the combination that will give the best results for the protein being studied. This overview unit discusses expression and production choices, including post‐translational modifications (e.g., glycosylation, acylation, sulfation, and removal of N‐terminal methionine), in vivo and in vitro folding, and influence of downstream elements on expression.

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Table of Contents

  • Background
  • Expression Systems
  • Post‐Translational Modifications and Expression System Choice
  • Production of Soluble Proteins by Host Cells
  • Protein‐Based Features
  • Special Considerations
  • Conclusions
  • Figures
  • Tables
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Internet Resources
  U.S. Food and Drug Administration Web site. Provides governmental information on replacing animal‐derived products with non‐animal products when using expressed protein for human clinical applications.
  European site providing similar information.
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