Light Scattering

Stephen E. Harding1, Kornelia Jumel1

1 University of Nottingham School of Biology, Sutton, Bonington
Publication Name:  Current Protocols in Protein Science
Unit Number:  Unit 7.8
DOI:  10.1002/0471140864.ps0708s11
Online Posting Date:  May, 2001
GO TO THE FULL TEXT: PDF or HTML at Wiley Online Library

Abstract

Light scattering methods can provide information about the native molecular weight, oligomeric composition, and gross conformation of a protein in solution. These methods are particularly well suited for studying large oligomeric systems or glycoproteins and can be used to characterize much larger structures involving protein such as viruses and even bacterial spores. This overview discusses theory and application of both static and dynamic light scattering analysis of protein solutions.

     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Table of Contents

  • Static Light Scattering Analysis of Protein Solutions
  • Dynamic Light Scattering Analysis of Protein Solutions
  • Figures
     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Materials

GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Figures

Videos

Literature Cited

Literature Cited
   Bahls, D.M. and Bloomfield, V.A. 1977. Turbidimetric determination of bacteriophage molecular weights. Biopolymers 16:2797‐2799.
   Brown, W. (ed.) 1993. Dynamic Light Scattering: The Method and Some Applications. Clarendon Press, Oxford.
   Burchard, W. 1992. Static and dynamic light scattering approaches to structure determination of biopolymers. In Laser Light Scattering in Biochemistry (S.E. Harding, D.B. Sattelle, and V.A. Bloomfield, eds.) pp. 3‐22. Royal Society of Chemistry, London.
   Claes, P., Dunford, M., Kenney, A., and Vardy, P. 1992. An on‐line dynamic light scattering instrument for macromolecular characterisation. In Laser Light Scattering in Biochemistry (S.E. Harding, D.B. Sattelle, and V.A. Bloomfield, eds.) pp. 66‐76. Royal Society of Chemistry, London.
   Garcia de la Torre, J., Carrasco, B., and Harding, S.E. 1997. SOLPRO: Theory and computer program for the prediction of SOLution PROperties of rigid macromolecules and bioparticles. Evr. Biophys. J. 25:361‐372.
   Harding, S.E. 1997. Microbial laser light scattering. Biotechnol. Genet. Eng. Rev. 14:145‐164.
   Harding, S.E., Horton, J.C., and Colfen, H. 1997. The ELLIPS suite of macromolecular conformation algorithms. Eur. Biophys. J. 25:347‐359.
   Johnsen, R.M. and Brown, W. 1992. An overview of current methods of analysing QLS data. In Laser Light Scattering in Biochemistry (S.E. Harding, D.B. Sattelle, and V.A. Bloomfield, eds.) pp 77‐91. Royal Society of Chemistry, London.
   Johnson, P. 1993. Light scattering in the study of colloidal and macromolecular systems. Int. Rev. Phys. Chem. 12:61‐87.
   Johnson, P. and McKenzie, G.H. 1977. A laser light scattering study of haemoglobin systems. Proc. R. Soc. Lond. B. 199:263‐278.
   Johnson, P. and Ottewill, R.H. 1954. A light scattering study of diptheria toxin‐antitoxin interaction. Disc. Faraday Soc. 18:327‐337.
   Jumel, K., Browne, P., and Kennedy, J.F. 1992. The use of low angle laser light scattering with gel permeation chromatography for the molecular weight determination of biomolecules. In Laser Light Scattering in Biochemistry (S.E. Harding, D.B. Sattelle, and V.A. Bloomfield, eds.) pp. 23‐34. Royal Society of Chemistry, London.
   Jumel, K., Fogg, F.J.J., Hutton, D.A., Pearson, J.P., Allen, A., and Harding, S.E. 1997. A polydisperse linear random coil model for the quaternary structure of pig colonic mucin. Eur. Biophys. J. 25:477‐480.
   Langley, K.H. 1992. Developments in electrophoretic laser light scattering and some biochemical applications. In Laser Light Scattering in Biochemistry (S.E. Harding, D.B. Sattelle, and V.A. Bloomfield, eds.) pp. 151‐160. Royal Society of Chemistry, London.
   McNeil‐Watson, F.K. and Parker, A. 1992. New tools for biochemists: Combined laser Doppler microelectrophoresis and photon correlation spectroscopy. In Laser Light Scattering in Biochemistry (S.E. Harding, D.B. Sattelle, and V.A. Bloomfield, eds.), pp. 59‐65. Royal Society of Chemistry, London.
   Pusey, P.N. 1974. Correlation and light beating spectroscopy. In Photon Correlation and Light‐Beating Spectroscopy (H.Z. Cummings and E.R. Pike, eds.) p. 387. Plenum, New York.
   Sanders, A.H. and Cannell, D.S. 1980. Techniques for light scattering from hemoglobin. In Light Scattering in Liquids and Macromolecular Solutions (V. Degiorgio, M. Corti, and M. Giglio, eds.) pp. 173‐182. Plenum, New York.
   Stacey, K.A. 1956. Light Scattering in Physical Chemistry. Butterworths, London.
   Svedberg, T. 1927. Nobelvortrag gehalten zu Stockholm am 19. Mai 1927. Kolloid‐chem. Beih. 26:230‐244.
   Tanford, C. 1961. Physical Chemistry of Macromolecules. Chapters 4 and 5. John Wiley & Sons, New York.
   Wells, C., Molina‐Garcia, A.D., Harding, S.E., and Rowe, A.J. 1990. Self‐interaction of dynein from Tetrahyamena cilia. J. Muscle Res. Cell Motil. 11:344‐350.
   Wyatt, P.J. 1992. Combined differential light scattering with various liquid chromatography separation techniques. In Laser Light Scattering in Biochemistry (S.E. Harding, D.B. Sattelle, V.A. Bloomfield, V.A. eds.) pp. 35‐58. Royal Society of Chemistry, London.
   Yau, W.W., Kirkland, J.J., and Bly, D.D. 1979. Modern Size Exclusion Chromatography. John Wiley & Sons, New York.
   Zimm, B.H. 1948. J. Chem. Phys. 16:1093‐1099.
Key References
   Harding, S.E., Sattelle, D.B. and Bloomfield, V.A. (eds.) 1992. Laser Light Scattering in Biochemistry. Royal Society of Chemistry, London.
   Huglin, M.B. (ed.) 1972. Light Scattering from Polymer Solutions. Academic Press, London.
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library