Autoradiography

Daniel C. Bundy1

1 Eastman Kodak Company, New Haven
Publication Name:  Current Protocols in Protein Science
Unit Number:  Unit 10.11
DOI:  10.1002/0471140864.ps1011s10
Online Posting Date:  May, 2001
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Abstract

This overview provides a thorough discussion of the theory and application of autoradiography for determining the spatial distribution of radioisotope‐labeled substances within a membrane or specimen material. The various types of film are discussed along with instructions on their correct handling. Quantitative autoradiography is described as a means of calibrating experimental results. Importantly, specific guidelines are given for pre‐flashing film, along with exposure and processing procedures.

     
 
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Table of Contents

  • Types of Film
  • Handling of Autoradiography Film
  • Quantitative Autoradiography
  • Exposure Procedures
  • Preflashing the Film
  • Film‐Processing Recommendations
  • Tables
     
 
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Materials

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Literature Cited

Literature Cited
   Hamilton, J.F. 1977. The mechanism of formation of the latent image. In The Theory of the Photographic Process, 4th ed. (T.H. James, ed.) pp. 105‐145. Eastman Kodak Company, Rochester, N.Y.
   Laboratory and Research Products. 1993. Autoradiographic Detection Principles. Eastman Kodak Company, Rochester, New York.
   Laskey, R.A. and Mills, A.D. 1975. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur. J. Biochem. 56:335‐341.
   Steinfeld, R., McLaughlin, W., Vizard, D., and Bundy, D. 1994. Advances in autoradiography: A new film brings quality improvements. In The Biotechnology Report pp. 147‐149. Campden Publishing Ltd., London.
   Vizard, D., Attwood, J., and McLaughlin, W. 1996. A universal intensifying screen system for enhanced detection of low‐ and high energy isotopes. Am. Biotechnol. Lab. 14(11):74‐76.
Internet Resources
   http://www.kodak.com:80/daiHome/SIS/ksis.shtml
  Site dedicated to scientific imaging systems and autoradiography products. Contains technical information, analysis software demos, and sales and service information for all Kodak Scientific Imaging Systems products.
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