Principles of Macromolecular X‐Ray Crystallography

Alison B. Hickman1, David R. Davies1

1 National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda
Publication Name:  Current Protocols in Protein Science
Unit Number:  Unit 17.3
DOI:  10.1002/0471140864.ps1703s10
Online Posting Date:  May, 2001
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X‐ray crystallography is one of the major tools available for protein structural analysis. This unit provides an introductory review of the principles of X‐ray crystallography that covers how the image is generated and analyzed. The second half of the unit describes strategies for producing crystals of protein, including methods for dealing with proteins that do not easily form crystals.

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Table of Contents

  • Crystal Structure Determination by X‐Ray Diffraction
  • Procedures for Crystallization
  • Figures
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Literature Cited

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Internet Resources
  Hampton Research Home Page; discusses aspects of protein crystallography; contains links to other Web sites.
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