Storage and Handling Guidelines for Custom Peptides

Amanda Turner1, Katy Radburn‐Smith1, Adeel Mushtaq1, Linda Tan1

1 thinkpeptides, ProImmune Ltd, Oxford, United Kingdom
Publication Name:  Current Protocols in Protein Science
Unit Number:  Unit 18.12
DOI:  10.1002/0471140864.ps1812s64
Online Posting Date:  April, 2011
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Abstract

Synthetic custom peptides offer a rational, scalable, and ever more affordable approach for exploring protein‐protein interactions or even more complex phenomena, such as immune responses directed against specific epitopes. As the use of such peptides increases, it becomes increasingly important to consider all relevant factors when designing and using peptides. This unit aims to provide guidelines for the storage and handling of custom peptides, to enable the user to achieve optimal results when using peptides in their experiments. It also discusses the implications of the presence of certain amino acids in the peptide sequence and suggests design considerations to help achieve desired outcomes. Curr. Protoc. Protein Sci. 64:18.12.1‐18.12.7. © 2011 by John Wiley & Sons, Inc.

Keywords: peptide; custom peptide; storage; solubility; concentration; aliquot; design; purity

     
 
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Table of Contents

  • Introduction
  • Literature Cited
  • Tables
     
 
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Materials

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Figures

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Literature Cited

Literature Cited
   Edelhoch, H., 1967. Spectroscopic determination of tryptophan and tyrosine in proteins. Biochemistry 6:1948‐1954.
   Gill, S.C. and von Hippel, P.H., 1989. Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182:319‐326.
   Kyte, J. and Doolittle, R.F. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:105‐132.
   Scopes, R.K. 1974. Measurement of protein by spectrophotometry at 205 nm. Anal. Biochem. 59:277‐282.
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