Survey of Protein Engineering Strategies

Uwe Bornscheuer1, Romas J. Kazlauskas2

1 Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Greifswald, Germany, 2 Department of Biochemistry, Molecular Biology and Biophysics and the Biotechnology Institute, University of Minnesota, Saint Paul, Minnesota
Publication Name:  Current Protocols in Protein Science
Unit Number:  Unit 26.7
DOI:  10.1002/0471140864.ps2607s66
Online Posting Date:  November, 2011
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Protein engineering is altering the structure of a protein to improve or change its properties. This unit summarizes concepts for protein engineering using rational design, directed evolution, and combinations of them. Different strategies are presented for identifying the best mutagenesis method, how to identify desired variants by screening or selection, and examples for successful applications are given. This should enable researchers to choose the most promising tools to solve their protein engineering challenges. Curr. Protoc. Protein Sci. 66:26.7.1‐26.7.14. © 2011 by John Wiley & Sons, Inc.

Keywords: protein engineering; rational protein design; directed evolution; biocatalysis

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Table of Contents

  • Introduction
  • Current Strategies to Engineer Proteins
  • Strategies to Engineer Specific Properties
  • Mutagenesis Methods
  • Conclusions
  • Acknowledgements
  • Literature Cited
  • Figures
  • Tables
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Key References
  Lutz and Bornscheuer, 2009. See above.
  This two volume edited book comprehensively covers current protein engineering methods.
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