Overview of Protein Microarrays

F.X. Reymond Sutandy1, Jiang Qian2, Chien‐Sheng Chen1, Heng Zhu3

1 Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli, Taiwan, 2 The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Baltimore, Maryland, 3 High‐Throughput Biology Center, Johns Hopkins School of Medicine, Baltimore, Maryland
Publication Name:  Current Protocols in Protein Science
Unit Number:  Unit 27.1
DOI:  10.1002/0471140864.ps2701s72
Online Posting Date:  April, 2013
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Protein microarray technology is an emerging field that provides a versatile platform for the characterization of hundreds of thousands of proteins in a highly parallel and high‐throughput manner. Protein microarrays are composed of two major classes: analytical and functional. In addition, tissue or cell lysates can also be fractionated and spotted on a slide to form a reverse‐phase protein microarray. Applications of protein microarrays, especially functional protein microarrays, have flourished over the past decade as the fabrication technology has matured. In this unit, advances in protein microarray technologies are reviewed, and then a series of examples are presented to illustrate the applications of analytical and functional protein microarrays in both basic and clinical research. Relevant areas of research include the detection of various binding properties of proteins, the study of protein post‐translational modifications, the analysis of host‐microbe interactions, profiling antibody specificity, and the identification of biomarkers in autoimmune diseases. Curr. Protoc. Protein Sci. 72:27.1.1‐27.1.16. © 2013 by John Wiley & Sons, Inc.

Keywords: protein microarrays; PTM; biomarker; network; systems biology

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Table of Contents

  • Introduction
  • Types of Protein Microarrays
  • Fabrication of Protein Microarrays
  • Detection
  • Applications in Basic Research
  • Applications in Clinical Research
  • Future Prospects
  • Acknowledgements
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

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