Introduction to Membrane Proteins

Mark L. Chiu1

1 Biologics Research, Biotechnology Center of Excellence, Janssen Research & Development, Radnor, Pennsylvania
Publication Name:  Current Protocols in Protein Science
Unit Number:  Unit 29.1
DOI:  10.1002/0471140864.ps2901s67
Online Posting Date:  February, 2012
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Membrane proteins (MPs) mediate important physiological processes for the cell via extracellular and intracellular interactions. To better understand the biochemical and structural bases of these interactions, well‐characterized preparations of purified MPs are required. This introduction reviews common problems encountered in MP preparation.

Keywords: membrane protein; purification and detergent solubilization; protein stabilization

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Table of Contents

  • Introduction
  • Expression
  • Purification
  • Characterization
  • Conclusions
  • Figures
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