Commonly Used Detergents

John E. Coligan1

1 National Institute of Allergy and Infectious Diseases, Bethesda, Maryland
Publication Name:  Current Protocols in Protein Science
Unit Number:  Appendix 1B
DOI:  10.1002/0471140864.psa01bs11
Online Posting Date:  May, 2001
GO TO THE FULL TEXT: PDF or HTML at Wiley Online Library

Abstract

This appendix lists some common detergents along with useful information regarding the type of hydrophilic group (anionic, cationic, amphoteric, or nonionic), the critical micelle concentration (CMC) and the micelle molecular weight.

     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Table of Contents

  • Types of Detergents
  • Choice of Detergents
  • Tables
     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Materials

GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Figures

Videos

Literature Cited

Literature Cited
   Furth, A.J., Bolton, H., Potter, J., and Priddle, J.D. 1984. Separating detergents from proteins. Methods Enzymol. 104:318‐328.
   Harlow, E. and Lane, D. 1988. Detergents. In Antibodies: A Laboratory Manual, pp. 687‐689. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
   Helenius, A., McCaslin, D.R., Fries, E., and Tanford, C. 1979. Properties of detergents. Methods Enzymol. 56:734‐749.
   Hjelmand, L.M. 1979. Removal of detergents from membrane proteins. Methods Enzymol. 182:277‐282.
   Jones, O.T., Earnest, J.P. and McNamee, M.G. 1987. Solubilization and reconstitution of membrane proteins. In Biological Membranes: A Practical Approach (J. Findlay and W.H. Evans, eds.), pp. 142‐143. IRL Press, Oxford.
Key References
   Harlow and Lane, E. 1988. See above.
  Provides properties of commonly used detergents and means of removing them from proteins.
   Hjelmeland, L.M. and Chrambach, A. 1984. Solubilization of functional membrane proteins. Methods Enzymol. 182:305‐318.
  Describes properties of detergents and how to use them to solubilize proteins.
   Johnstone, A. and Thorpe, R. 1982. Isolation and fractionation of lymphocytes. In Immunochemistry in Practice, pp. 94‐101. Blackwell Scientific, Oxford.
  Provides details in use of detergents to solubilize cells and membranes.
   Neugebaur, J.M. 1990. Detergents: An overview. Methods Enzymol. 182:239‐282.
  Provides details on detergent properties and choosing one for a particular application.
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library