Use of Protein Folding Reagents

Paul T. Wingfield1

1 Protein Expression Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS), NIH, Bethesda, Maryland
Publication Name:  Current Protocols in Protein Science
Unit Number:  Appendix 3A
DOI:  10.1002/0471140864.psa03as84
Online Posting Date:  April, 2016
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Abstract

The reagents and methods for purification and use of the most commonly used denaturants, guanidine hydrochloride (guanidine‐HCl) and urea, are described. Other protein denaturants and reagents used to fold proteins are briefly mentioned. Sulfhydryl reagents (reducing agents) and “oxido‐shuffling” (or oxidative regeneration) systems are also described. © 2016 by John Wiley & Sons, Inc.

Keywords: protein folding; guanidine hydrochloride; urea; protein denaturants; sulfhydryl reagents; oxidative regeneration

     
 
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Table of Contents

  • Denaturants
  • Sulfhydryl Reagents and Oxido‐Shuffling Systems
  • Tables
     
 
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Materials

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Literature Cited

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