Dialysis

Louis Zumstein1

1 Introgen Therapeutics, Inc., Houston, Texas
Publication Name:  Current Protocols in Protein Science
Unit Number:  Appendix 3B
DOI:  10.1002/0471140864.psa03bs00
Online Posting Date:  May, 2001
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Abstract

This appendix describes dialysis of large‐ and small‐volume samples using cellulose membranes with pore sizes designed to exclude molecules above a selected molecular weight. A support protocol describes preparation of membranes for dialysis.

     
 
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Table of Contents

  • Basic Protocol 1: Large‐Volume Dialysis
  • Alternate Protocol 1: Small‐Volume Dialysis
  • Support Protocol 1: Selection and Preparation of Dialysis Membrane
  • Literature Cited
     
 
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Materials

Basic Protocol 1: Large‐Volume Dialysis

  Materials
  • Dialysis membrane (see protocol 3)
  • Clamps (Spectrapor Closures, Spectrum, or equivalent)
  • Macromolecule‐containing sample to be dialyzed
  • Appropriate dialysis buffer

Alternate Protocol 1: Small‐Volume Dialysis

  Additional Materials
  • 0.5‐ml microcentrifuge tube
  • Cork borer

Support Protocol 1: Selection and Preparation of Dialysis Membrane

  Additional Materials
  • 10 mM sodium bicarbonate
  • 10 mM Na 2EDTA, pH 8.0
  • 20% to 50% (v/v) ethanol
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Figures

Videos

Literature Cited

Literature Cited
   Craig, L.C. 1967. Techniques for the study of peptides and proteins by dialysis and diffusion. Methods Enzymol. 11:870‐905.
  Describe the theory and practice of dialysis and diffusion.
   McPhie, P. 1971. Dialysis. Methods Enzymol. 22:23‐32.
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