Silanizing Glassware

Brian Seed1

1 Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts
Publication Name:  Current Protocols in Protein Science
Unit Number:  Appendix 3E
DOI:  10.1002/0471140864.psa03es13
Online Posting Date:  May, 2001
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Abstract

Untreated glass contains silicate and silanol groups that can act as ion‐exchange and nucleophilic centers. To mask these groups and decrease the hydrophilicity of the surface, various reactive silanes are frequently used to coat the glass surface. This appendix describes a procedure for silanizing glassware.

     
 
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Table of Contents

  • Commentary
     
 
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Materials

Basic Protocol 1:

  Materials
  • Chlorotrimethylsilane or dichlorodimethylsilane
  • Vacuum pump
  • Desiccator, equipped with a valve
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Figures

Videos

Literature Cited

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