Molecular Biology Techniques

Karen L. Elbing1, roger Brent2

1 Clark and Elbing LLP, Boston, 2 The Molecular Science Institute, Berkeley
Publication Name:  Current Protocols in Protein Science
Unit Number:  Appendix 4
DOI:  10.1002/0471140864.psa04s13
Online Posting Date:  May, 2001
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Abstract

Some of the units in this manual describe techniques for the cloning, expression, and structural analysis of genes and proteins. Users of these protocols are assumed to have at least some introductory background in recombinant DNA technology (or are working with a collaborator who does); therefore, comprehensive coverage of these topics is not provided, but selected techniques are presented that will be of the most interest and use to the general protein laboratory. More comprehensive coverage of these techniques can be found in Current Protocols in Molecular Biology or any of a variety of textbooks devoted to recombinant DNA techniques.

Keywords: molecular biology; methods; recombinant DNA

     
 
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Table of Contents

         
     
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    Materials

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    Literature Cited

    Key References
       Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (eds.). 1998. urrent Protocols in Molecular Biology. John Wiley and Sons, New York.
       Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular Cloning: A Laboratory, 2nd ed. Cold Spring Harbor Laboratory Harbor, N.Y., Cold Spring
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