Growth in Liquid or Solid Media

Karen L. Elbing1, Roger Brent2

1 Clark and Elbing LLP, Boston, 2 The Molecular Sciences Institute, Berkeley
Publication Name:  Current Protocols in Protein Science
Unit Number:  Appendix 4B
DOI:  10.1002/0471140864.psa04bs13
Online Posting Date:  May, 2001
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Abstract

This appendix presents basic procedures for growing overnight (and larger) cultures, monitoring growth, and titering and isolating bacterial cultures by serial dilution. In addition, protocols are provided for isolating single colonies by streaking and spreading a plate. Replica plating and strain storage/retrieval is also detailed.

     
 
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Table of Contents

  • Basic Protocol 1: Growing an Overnight Culture
  • Basic Protocol 2: Growing Larger Cultures
  • Basic Protocol 3: Monitoring Growth
  • Basic Protocol 4: Titering and Isolating Bacterial Colonies by Serial Dilutions
  • Basic Protocol 5: Isolating Single Colonies by Streaking a Plate
  • Basic Protocol 6: Isolating Single Colonies by Spreading a Plate
  • Support Protocol 1: Replica Plating
  • Support Protocol 2: Strain Storage and Revival
  • Figures
     
 
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Materials

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Figures

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Literature Cited

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