Transgenic Animals in Toxicology

Warren C. Ladiges1, Carol B. Ware1

1 University of Washington, Seattle, Washington
Publication Name:  Current Protocols in Toxicology
Unit Number:  Unit 1.3
DOI:  10.1002/0471140856.tx0103s00
Online Posting Date:  May, 2001
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Transgenic animals are a powerful tool in the exploration of the interaction of specific genes with the environment and/or toxins to understand the relation between biochemical and metabolic pathways and toxic effect. This overview discusses DNA construct design, methods for production of transgenic animals, including pronuclear fusion, gene targeting and chimera production, animal husbandry, and colony management.

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Table of Contents

  • Strategies and Applications
  • DNA Construct Considerations
  • Mouse Husbandry
  • Production of Tg Mice by Pronuclear Microinjection
  • Production of Tg Mice by Gene Targeting
  • Colony Management
  • Literature Cited
  • Figures
  • Tables
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Literature Cited

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Key References
   Davisson, M.T. 1994. Rules and guidelines for genetic nomenclature in mice. Mouse Genome 92(2):1‐32.
  Explains the details in providing Tg animals with a standard nomenclature and provides resources available for assistance.
   Hogan et al., 1994. See above.
  Classic handbook for overall mouse embryo techniques.
   Joyner, A.L. 1993. Gene Targeting: A Practical Approach. IRL Press, Oxford.
  Focuses on the protocols required for gene targeting by homologous recombination.
   Wassarman, P.M. and DePamphilis, M.L. (eds.) 1993. Guide to techniques in mouse development. Parts X and XI. Methods Enzymol. vol. 225.
  Overall presentation of transgenic techniques.
Internet Resources
  Web site for accessing TBASE, the transgenic/targeted mutation database administered by The Jackson Laboratory.‐list
  Web site to pick up past messages from the transgenic list discussion, which is an informal discussion on all aspects of transgenics.
  The transgenic web site at the University of Michigan, which contains access to a wide array of other websites of use in the pursuit of transgenics.
  E‐mail address to join the transgenics list discussion group. Message must include: subscribe transgenic‐list.
  E‐mail address for Dr. Lee Silver, to obtain information on the mouse colony management software Animal House Manager, which is a software package for keeping track of mouse breeding colonies.
  The Mouse Genome Informatics Web site at The Jackson Laboratory, which contains information on mouse genetic markers, molecular segments, phenotypes, comparative mapping data, experimental mapping data, and graphical displays for genetic, physical, and cytogenetic maps.
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