Detection of Metabolites Using High‐Performance Liquid Chromatography and Mass Spectrometry

Thomas D. McClure1

1 Novartis Agricultural Discovery Institute, Inc., San Diego, California
Publication Name:  Current Protocols in Toxicology
Unit Number:  Unit 4.4
DOI:  10.1002/0471140856.tx0404s03
Online Posting Date:  May, 2001
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Abstract

This unit provides a discussion of high‐pressure liquid chromatography‐atmospheric pressure ionization mass spectrometry (LC‐API‐MS) for separation, detection, and identification of products from xenobiotic metabolism. It includes an introduction and important technical information for electrospray and atmospheric pressure chemical ionization methods as well as scan modes. Emphasis has been placed on each method as related to phase I and phase II metabolite identification and there are listings of specific applications (and references) to reduce method development time.

     
 
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Table of Contents

  • Instrumental Considerations
  • Combined HPLC‐MS Methodology
  • HPLC‐MS and HPLC‐MS/MS of Metabolites
  • Summary
  • Future Directions
  • Figures
  • Tables
     
 
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Materials

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Literature Cited

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