Collection of Bile and Urine Samples for Determining the Urinary and Hepatobiliary Disposition of Xenobiotics in Mice
One of the most simple and informative method commonly used by researchers in the field of xenobiotics metabolism and dispostion to investigate the fate of chemicals in living organisms involves identification and/or quantitation of metabolites in excreta such as urine and bile. This method can be performed on dogs and larger rodents such as rats and hamsters rather easily. However, many investigators find it difficult to collect bile samples from mice. This unit describes, in detail, how to collect urine and bile samples from a mouse for further metabolite analysis.
Basic Protocol 1: Collection of Bile and Urine Samples from a Bile Duct–Cannulated Mouse
Figure 5.7.1 Diagram of the bile duct system in mice. Figure reproduced from Iwaki et al., () with permission of Braintree Scientific, Inc.
|Iwaki, T., Yamashita, H., and Hayakawa, T. 2001. A Color Atlas of Sectional Anatomy of the Mouse. Braintree Scientific, Inc. Braintree, Mass.|
|Kanz, M.F., Whitehead, R.F., Freguson, A.E., Kaphalia, L., and Moslen, M.T. 1992. Biliary function studies during multiple time periods in freely moving rats. A useful system and set of marker solutes. J. Pharmacol. Toxicol. Methods 27:7‐15.|