Scoring of Social Interactions and Play in Mice During Adolescence

M. Livia Terranova1, Giovanni Laviola1

1 Istituto Superiore di Sanità, Rome
Publication Name:  Current Protocols in Toxicology
Unit Number:  Unit 13.10
DOI:  10.1002/0471140856.tx1310s26
Online Posting Date:  December, 2005
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Abstract

This unit provides a description of methods that have proven useful in characterizing amicable and playful interactions of developing mice. Such a procedure can be used to evaluate the effects of perinatal and/or ongoing treatments on the social performance of periadolescent subjects of either or both sexes. It can also be complemented by the use of specific acute drug challenges, which can throw light on possible alterations of the subserving neurochemical systems. Basically, it consists of video recording brief sessions of spontaneous pair interactions and their subsequent observation and scoring according to a detailed mouse ethogram. The protocol is quite sensitive to subtle behavioral effects, which could be undetectable by other means, and it is most useful when repeated over several days to draw an ontogenetic profile. Critical parameters that must be considered when planning, e.g., sample size and timing of observations, are discussed in detail, along with the key issue of controlling for litter effects.

Keywords: Behavior; Ontogeny; Affiliation; Critical periods; Rodents

     
 
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Table of Contents

  • Strategic Planning
  • Basic Protocol 1: Video Recording of Social Encounters
  • Basic Protocol 2: Observational Assessments
  • Commentary
  • Literature Cited
     
 
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Materials

Basic Protocol 1: Video Recording of Social Encounters

  Materials
  • Periadolescent mice (from 21 to 45 postnatal days)
  • Isolated, soundproof experimental room equipped with red lights
  • Observation cages (standard housing cages with tops)
  • Standard sawdust
  • 2 to 3 small movable objects (e.g., food pellets)
  • Infrared video cameras, video recorders, monitors, and tapes
  • Event recorder system feeding to a computer with appropriate software
  • Balance for weighing animals
  • Nontoxic marker for subject identification
  • Paper labels for code identification
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Figures

Videos

Literature Cited

Literature Cited
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