Guidelines for Mating Rodents

Rochelle W. Tyl1

1 Center of Life Sciences and Toxicology Research Triangle Institute, Research Triangle Park, North Carolina
Publication Name:  Current Protocols in Toxicology
Unit Number:  Unit 16.2
DOI:  10.1002/0471140856.tx1602s11
Online Posting Date:  May, 2002
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Abstract

Guidelines for Mating Rodents (Rochelle W. Tyl, Research Triangle Institute, Research Triangle Park, North Carolina). Mating is an important part of assessing the effects of toxicants on the reproductive tract. This unit describes the set up of mating pairs, determining the success of mating, and assessing the various outcomes of a successful mating to evaluate reproductive function.

     
 
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Table of Contents

  • Basic Protocol 1: Determination of Estrous Cyclicity in Rodents
  • Basic Protocol 2: Rodent Mating
  • Basic Protocol 3: Gestation and Lactation in Rats and Mice
  • Literature Cited
  • Tables
     
 
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Materials

Basic Protocol 1: Determination of Estrous Cyclicity in Rodents

  Materials
  • Female rat or mouse
  • Saline: 0.9% (w/v) NaCl, sterile ( appendix 2A)
  • Spray‐Cyte fixative (Clay Adam)
  • 50%, 70%, 80%, 95%, and 100% (v/v) ethanol
  • 1% (w/v) toluidine blue/1% (w/v) sodium borate
  • Xylene
  • Permount (Fisher)
  • Fire‐polished eye dropper
  • Glass microscope slide prelabeled with a six‐cell grid using a permanent marker (e.g., Securline Marker II/Superfrost; Precision Dynamics)
  • Coverslips
CAUTION: Xylene is toxic and should be used in a fume hood.

Basic Protocol 2: Rodent Mating

  Materials
  • Male and female mice or rats
  • Saline: 0.9% (w/v) NaCl, sterile ( appendix 2A), for rats only
  • Cages, for mating (with either a solid bottom and contact bedding or a wire bottom)
  • Plastic cages, for mated females (with a solid bottom and contact bedding)
  • Fire‐polished eyedropper, for rats only
  • Glass microscope slides, for rats only
  • Microscope (with 10× and 40× eyepieces), for rats only
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Figures

Videos

Literature Cited

Literature Cited
   Environmental Protection Agency (EPA). 1998. Health Effects Test Guidelines, Report No. OPPTS 870.3700, Prenatal Developmental Toxicity Study. Office of Prevention, Pesticides and Toxic Substances (OPPTS), U.S. EPA, Washington, D.C.
   European Society for Human Reproduction and Embryology (ESHRE) Andrology Special Interest Group. 1998. Guidelines on the application of CASA technology in the analysis of spermatozoa. Hum. Reprod. 13:142‐145.
   Haas, E. 1981. Fifty Diagnostic Special Stains for Surgical Pathology p.114. Lippincott, Philadelphia.
   Organization for Economic Cooperation and Development (OECD). 2001. Proposal for Updating Guideline 414: Prenatal Developmental Toxicity Study, In Guideline for Testing of Chemicals (adopted January 22, 2001) pp. 1‐11. OECD, Paris
   Seed, J., Chapin, R.E., Clegg, E.D., Dostal, L.A., Foote, R.H., Hurtt, M.E., Klinefelter, G.R., Makris, S.L., Perreault, S.D., Schrader, S., Seyler, D., Sprando, R., Treinen, K.A., Veeramacheneni, D.N., and Wise, L.D. 1996. Methods for assessing sperm motility, morphology, and counts in the rat, rabbit, and dog: A consensus report. Reprod. Toxicol. 10:237‐244.
   Whitten, W.K. 1956. Modification of the oestrus cycle of the mouse by external stimuli associated with the male. J. Endocrinol. 13:399‐404.
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