Common Stock Solutions and Buffers


Publication Name:  Current Protocols in Toxicology
Unit Number:  Appendix 2A
DOI:  10.1002/0471140856.txa02as00
Online Posting Date:  May, 2001
GO TO THE FULL TEXT: PDF or HTML at Wiley Online Library

Abstract

    Recipes

  • Acid, concentrated stock solutions

  • Ammonium acetate, 10 M

  • Ammonium hydroxide, concentrated stock solution

  • Ammonium sulfate, saturated

  • ATP, 100 mM

  • Base, concentrated stock solutions

  • CaCl2, 1 M

  • Carbonate buffer

  • CMF‐DPBS (calcium‐ and magnesium‐free Dulbecco's phosphate‐buffered saline)

  • DPBS (Dulbecco's phosphate‐buffered saline)

  • DTT (dithiothreitol), 1 M

  • EDTA (ethylenediaminetetraacetic acid), 0.5 M (pH 8.0)

  • HBSS (Hanks' balanced salt solution)

  • HCl, 1 M

  • KCl, 1 M

  • MgCl2, 1 M

  • MgSO4, 1 M

  • NaCl, 5 M

  • NaCl (saline), 0.9% (w/v)

  • NaOH, 10 M

  • PBS (phosphate‐buffered saline)

  • Potassium acetate buffer, 0.1 M

  • Potassium phosphate buffer, 0.1 M

  • SDS, 20% (w/v)

  • SDS electrophoresis buffer, 5×

  • SDS sample buffer

  • Sodium acetate, 3 M

  • Sodium acetate buffer, 0.1 M

  • Sodium phosphate buffer, 0.1 M

  • TBS (Tris‐buffered saline)

  • Tris⋅Cl, 1 M

     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Table of Contents

  • Recipes
  • Literature Cited
  • Tables
     
 
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Materials

GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library

Figures

Videos

Literature Cited

Literature Cited
   American Chemical Society (ACS). 1999. LabGuide 98‐99. ACS, Washington, D.C. [also available online at http://pubs.acs.org./labguide].
   Chemical Rubber Company (CRC). 1975. CRC Handbook of Biochemistry and Molecular Biology, Physical and Chemical Data, 3d ed., Vol. 1. CRC Press, Boca Raton, Fla.
GO TO THE FULL PROTOCOL:
PDF or HTML at Wiley Online Library