Manipulating Gene Expression

Listings: 1-20   21-40   41-52
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Optimized PEI‐based Transfection Method for Transient Transfection and Lentiviral Production
Shaozhe Yang, Xiaoling Zhou, Rongxiang Li, Xiuhong Fu, Pingnan Sun
Published online: September 2017
A Guide to Creating and Testing New INTRSECT Constructs
Lief E. Fenno, Joanna Mattis, Charu Ramakrishnan, Karl Deisseroth
Published online: July 2017
Correlative Fluorescence and Electron Microscopy in 3D—Scanning Electron Microscope Perspective
Jonathan Franks, Callen T. Wallace, Masateru Shibata, Mitsuo Suga, Natasha Erdman, Donna B. Stolz, Simon C. Watkins
Published online: April 2017
CRISPR/Cas9‐Mediated Gene Knockout in the Mouse Brain Using In Utero Electroporation
Yohei Shinmyo, Hiroshi Kawasaki
Published online: April 2017
LOVTRAP: A Versatile Method to Control Protein Function with Light
Hui Wang, Klaus M. Hahn
Published online: December 2016
Use of Adeno‐Associated and Herpes Simplex Viral Vectors for In Vivo Neuronal Expression in Mice
Rachel D. Penrod, Audrey M. Wells, William A. Carlezon, Christopher W. Cowan
Published online: October 2015
CRISPR/Cas9‐Directed Genome Editing of Cultured Cells
Luhan Yang, Joyce L. Yang, Susan Byrne, Joshua Pan, George M. Church
Published online: July 2014
Developmental Toxicity Assays Using the Drosophila Model
Matthew D. Rand, Sara L. Montgomery, Lisa Prince, Daria Vorojeikina
Published online: February 2014
Isolation of Bacteriophages from Environmental Sources, and Creation and Functional Screening of Phage DNA Libraries
Adam J. Pelzek, Raymond Schuch, Jonathan E. Schmitz, Vincent A. Fischetti
Published online: October 2013
Protein Knockouts in Living Eukaryotes Using deGradFP and Green Fluorescent Protein Fusion Targets
Emmanuel Caussinus, Oguz Kanca, Markus Affolter
Published online: September 2013
Oncolytic Adenoviruses: Design, Generation, and Experimental Procedures
Julia Davydova, Masato Yamamoto
Published online: July 2013
Spatiotemporal Control of Gene Expression in Mammalian Cells and in Mice Using the LightOn System
Xianjun Chen, Xue Wang, Zengmin Du, Zhengcai Ma, Yi Yang
Published online: June 2013
A Detailed Protocol for Formaldehyde‐Assisted Isolation of Regulatory Elements (FAIRE)
Jeremy M. Simon, Paul G. Giresi, Ian J. Davis, Jason D. Lieb
Published online: April 2013
Genetic Manipulation of Human Induced Pluripotent Stem Cells
Angela Wang, Chee Gee Liew
Published online: November 2012
Chick Embryo Culture and Electroporation
Yukinori Endo
Published online: September 2012
Transfection by Electroporation
Huntington Potter, Richard Heller
Published online: September 2011
Basic Techniques in Human Mesenchymal Stem Cell Cultures: Differentiation into Osteogenic and Adipogenic Lineages, Genetic Perturbations, and Phenotypic Analyses
Claudia Bruedigam, Marjolein van Driel, Marijke Koedam, Jeroen van de Peppel, Bram C.J. van der Eerden, Marco Eijken, Johannes P.T.M. van Leeuwen
Published online: June 2011
Lentiviral Vector Mediated Transgenesis
Isabelle Barde, Sonia Verp, Sandra Offner, Didier Trono
Published online: March 2011
Listings: 1-20   21-40   41-52