Molecular Biology Techniques

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Protein Blotting: Immunoblotting
Duojiao Ni, Peng Xu, Diviya Sabanayagam, Sean R. Gallagher
Published online: May 2016
RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian Cells
Grigory S. Filonov, Samie R. Jaffrey
Published online: March 2016
Site‐Specific Recombinational Cloning Using Gateway and In‐Fusion Cloning Schemes
Jaehong Park, Andrea L. Throop, Joshua LaBaer
Published online: April 2015
Mapping Regulatory Factors by Immunoprecipitation from Native Chromatin
Guillermo A. Orsi, Sivakanthan Kasinathan, Gabriel E. Zentner, Steven Henikoff, Kami Ahmad
Published online: April 2015
SNIPER Peptide‐Mediated Degradation of Endogenous Proteins
Xuelai Fan, Yu Tian Wang
Published online: March 2015
Noninvasive Prenatal Testing Using Cell‐Free Fetal DNA in Maternal Plasma
Nilesh Dharajiya, Tricia Zwiefelhofer, Xiaojun Guan, Vach Angkachatchai, Juan‐Sebastian Saldivar
Published online: January 2015
BioTAP‐XL: Cross‐linking/Tandem Affinity Purification to Study DNA Targets, RNA, and Protein Components of Chromatin‐Associated Complexes
Artyom A. Alekseyenko, Kyle A. McElroy, Hyuckjoon Kang, Barry M. Zee, Peter V. Kharchenko, Mitzi I. Kuroda
Published online: January 2015
Mapping Replication Origin Sequences in Eukaryotic Chromosomes
Haiqing Fu, Emilie Besnard, Romain Desprat, Michael Ryan, Malik Kahli, Jean‐Marc Lemaitre, Mirit I. Aladjem
Published online: December 2014
CRISPR/Cas9‐Directed Genome Editing of Cultured Cells
Luhan Yang, Joyce L. Yang, Susan Byrne, Joshua Pan, George M. Church
Published online: July 2014
Isolation, Culture, and Transient Transformation of Plant Protoplasts
Jinbo Shen, Jiaxin Fu, Jin Ma, Xiangfeng Wang, Caiji Gao, ChuXiong Zhuang, Jianmin Wan, Liwen Jiang
Published online: June 2014
Rapid Inactivation of Proteins by Knocksideways
Margaret S. Robinson, Jennifer Hirst
Published online: December 2013
Expanding Mouse Ventricular Cardiomyocytes Through GSK‐3 Inhibition
Jan W. Buikema, Peter‐Paul M. Zwetsloot, Pieter A. Doevendans, Joost P.G. Sluijter, Ibrahim J. Domian
Published online: December 2013
RAMPAGE: Promoter Activity Profiling by Paired‐End Sequencing of 5′‐Complete cDNAs
Philippe Batut, Thomas R. Gingeras
Published online: November 2013
Methods to Detect Protein Glutathionylation
Robyn L. Poerschke, Kristofer S. Fritz, Christopher C. Franklin
Published online: September 2013
Oncolytic Adenoviruses: Design, Generation, and Experimental Procedures
Julia Davydova, Masato Yamamoto
Published online: July 2013
RNA Isolation from Mammalian Samples
Ximeng Liu, Shuko Harada
Published online: July 2013
Genome‐Wide Annotation and Quantitation of Translation by Ribosome Profiling
Nicholas T. Ingolia, Gloria A. Brar, Silvia Rouskin, Anna M. McGeachy, Jonathan S. Weissman
Published online: July 2013
Spatiotemporal Control of Gene Expression in Mammalian Cells and in Mice Using the LightOn System
Xianjun Chen, Xue Wang, Zengmin Du, Zhengcai Ma, Yi Yang
Published online: June 2013
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