Molecular Biology Techniques

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Spatiotemporal Control of Gene Expression in Mammalian Cells and in Mice Using the LightOn System
Xianjun Chen, Xue Wang, Zengmin Du, Zhengcai Ma, Yi Yang
Published online: June 2013
Insertion of a GFP Reporter Gene in Influenza Virus
Jasmine T. Perez, Adolfo García‐Sastre, Balaji Manicassamy
Published online: May 2013
Poliovirus: Generation, Quantification, Propagation, Purification, and Storage
Cecily P. Burrill, Vanessa R. Strings, Raul Andino
Published online: May 2013
DNA Isolation from Mammalian Samples
Ximeng Liu, Shuko Harada
Published online: April 2013
A Detailed Protocol for Formaldehyde‐Assisted Isolation of Regulatory Elements (FAIRE)
Jeremy M. Simon, Paul G. Giresi, Ian J. Davis, Jason D. Lieb
Published online: April 2013
Analysis of Interactions Between Genomic Loci Through Chromosome Conformation Capture (3C)
Belal El Kaderi, Scott Medler, Athar Ansari
Published online: September 2012
Approaches to Studying Arf GAPs in Cells: In Vitro Assay with Isolated Focal Adhesions
Pei‐Wen Chen, Xiaoying Jian, Ruibai Luo, Paul A. Randazzo
Published online: June 2012
Analysis of Nonsense‐Mediated mRNA Decay in Mammalian Cells
Pamela Nicholson, Raphael Joncourt, Oliver Mühlemann
Published online: June 2012
Use of Zinc Finger Nuclease Technology to Knock Out Efflux Transporters in C2BBe1 Cells
Jennifer Pratt, Neetu Venkatraman, Amanda Brinker, Yongling Xiao, Jim Blasberg, David C. Thompson, Maureen Bourner
Published online: May 2012
Preparation of Genomic DNA from Mammalian Sperm
Alexandra Weyrich
Published online: April 2012
Analysis of Nonsense‐Mediated mRNA Decay in Saccharomyces cerevisiae
Bessie W. Kebaara, Kristian E. Baker, Krista D. Patefield, Audrey L. Atkin
Published online: March 2012
Larry A. McReynolds, Nicole M. Nichols
Published online: October 2011
Transfection by Electroporation
Huntington Potter, Richard Heller
Published online: October 2011
Stem‐Loop RT‐qPCR for miRNAs
Martha F. Kramer
Published online: July 2011
DNA Ligases
Gregory J.S. Lohman, Stanley Tabor, Nicole M. Nichols
Published online: April 2011
COS‐1 Cells as Packaging Host for Production of Lentiviruses
Crystal J. MacKenzie, Toshi Shioda
Published online: March 2011
Production of Lentiviral Vectors in Protein‐free Media
Hitoshi Kuroda, Michael P. Marino, Robert H. Kutner, Jakob Reiser
Published online: March 2011
Monitoring Viral‐Mediated Membrane Fusion Using Fluorescent Reporter Methods
Naoyuki Kondo, Kosuke Miyauchi, Zene Matsuda
Published online: March 2011
Imaging of Endogenous RNA Using Genetically Encoded Probes
Takeaki Ozawa, Yoshio Umezawa
Published online: February 2011
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