Extraction, Stabilization, and Concentration
Overview of Approaches to Preventing and Avoiding Proteolysis During Expression and Purification of Proteins
Barry J. Ryan, Gary T. Henehan
Published online: February 2013
Barry J. Ryan, Gary T. Henehan
Published online: February 2013
Preparation and Extraction of Insoluble (Inclusion‐Body) Proteins from Escherichia coli
Ira Palmer, Paul T. Wingfield
Published online: November 2012
Ira Palmer, Paul T. Wingfield
Published online: November 2012
Removal of Detergents from Proteins and Peptides in a Spin‐Column Format
Babu S. Antharavally
Published online: August 2012
Babu S. Antharavally
Published online: August 2012
Overexpression of Membrane Proteins Using Pichia pastoris
Olivier Bornert, Fatima Alkhalfioui, Christel Logez, Renaud Wagner
Published online: February 2012
Olivier Bornert, Fatima Alkhalfioui, Christel Logez, Renaud Wagner
Published online: February 2012
Purification of the Human G Protein−Coupled Receptor Adenosine A2aR in a Stable and Functional Form Expressed in Pichia pastoris
Shweta Singh, Minghao Zhang, Nicolas Bertheleme, Philip G. Strange, Bernadette Byrne
Published online: February 2012
Shweta Singh, Minghao Zhang, Nicolas Bertheleme, Philip G. Strange, Bernadette Byrne
Published online: February 2012
In Vitro Transcription and Immobilized Template Analysis of Preinitiation Complexes
Justin J. Lin, Michael Carey
Published online: January 2012
Justin J. Lin, Michael Carey
Published online: January 2012
Extraction, Purification, and Identification of Yersiniabactin, the Siderophore of Yersinia pestis
M. Clarke Miller, Edward DeMoll
Published online: November 2011
M. Clarke Miller, Edward DeMoll
Published online: November 2011
The Combined Use of the Thermofluor Assay and ThermoQ Analytical Software for the Determination of Protein Stability and Buffer Optimization as an Aid in Protein Crystallization
Kevin Phillips, Andres Hernandez de la Peña
Published online: April 2011
Kevin Phillips, Andres Hernandez de la Peña
Published online: April 2011
Isolation of Proteins by Heat‐Induced Extraction from Formalin‐Fixed, Paraffin‐Embedded Tissue and Preparation of Tryptic Peptides for Mass Spectrometric Analysis
Yuan Tian, Hui Zhang
Published online: April 2010
Yuan Tian, Hui Zhang
Published online: April 2010
Extraction of Proteins from Formalin‐Fixed, Paraffin‐Embedded Tissue Using the Qproteome Extraction Technique and Preparation of Tryptic Peptides for Liquid Chromatography/Mass Spectrometry Analysis
Thibault Geoui, Henning Urlaub, Uwe Plessmann, Peter Porschewski
Published online: April 2010
Thibault Geoui, Henning Urlaub, Uwe Plessmann, Peter Porschewski
Published online: April 2010
Methods of Identifying Membrane Proteins in Spirochetes
James A. Carroll
Published online: February 2010
James A. Carroll
Published online: February 2010
Isolation and Biochemical Characterization of Amyloid Plaques and Paired Helical Filaments
Agueda Rostagno, Jorge Ghiso, Jorge Ghiso
Published online: September 2009
Agueda Rostagno, Jorge Ghiso, Jorge Ghiso
Published online: September 2009
Overview of Chromatography
Buddhadeb Mallik, Bulbul Chakravarti, Deb N. Chakravarti
Published online: October 2008
Buddhadeb Mallik, Bulbul Chakravarti, Deb N. Chakravarti
Published online: October 2008
The Use of Detergents to Purify Membrane Proteins
Thomas Arnold, Dirk Linke
Published online: August 2008
Thomas Arnold, Dirk Linke
Published online: August 2008
Preparation of Nuclear and Cytoplasmic Extracts from Mammalian Cells
Susan M. Abmayr, Tingting Yao, Tari Parmely, Jerry L. Workman
Published online: August 2006
Susan M. Abmayr, Tingting Yao, Tari Parmely, Jerry L. Workman
Published online: August 2006
Preparation of Soluble Proteins from Escherichia coli
Paul T. Wingfield
Published online: September 2005
Paul T. Wingfield
Published online: September 2005
Desalting, Concentration, and Buffer Exchange by Dialysis and Ultrafiltration
Allen T. Phillips, Mark W. Signs
Published online: January 2005
Allen T. Phillips, Mark W. Signs
Published online: January 2005
Extraction of Proteins from Plant Tissues
William Laing, John Christeller
Published online: November 2004
William Laing, John Christeller
Published online: November 2004



