Current Protocols Essential Laboratory Techniques

Table of Contents

•   Common Conversion Factors
•   Combining Techniques to Answer Molecular Questions
• Chapter 1 Volume/Weight Measurement
  • Unit 1.1 Volume Measurement
  • Unit 1.2 Weight Measurement
• Chapter 2 Concentration Measurement
  • Unit 2.1 Spectrophotometry
  • Unit 2.2 Quantitation of Nucleic Acids and Proteins
  • Unit 2.3 Radiation Safety and Measurement
  • Unit 2.4 Fluorescence Spectroscopy
• Chapter 3 Reagent Preparation
  • Unit 3.1 Reagent Preparation: Theoretical and Practical Discussions
  • Unit 3.2 Measurement of pH
  • Unit 3.3 Recipes for Commonly Encountered Reagents
• Chapter 4 Cell Culture Techniques
  • Unit 4.1 Aseptic Technique
  • Unit 4.2 Culture of Escherichia coli and Related Bacteria
  • Unit 4.3 Mammalian Cell Culture
• Chapter 5 Sample Preparation
  • Unit 5.1 Centrifugation
  • Unit 5.2 Purification and Concentration of Nucleic Acids
• Chapter 6 Chromatography
  • Unit 6.1 Overview of Chromatography
  • Unit 6.2 Column Chromatography
• Chapter 7 Electrophoresis
  • Unit 7.1 Overview of Electrophoresis
  • Unit 7.2 Agarose Gel Electrophoresis
  • Unit 7.3 SDS‐Polyacrylamide Gel Electrophoresis (SDS‐PAGE)
  • Unit 7.4 Staining Proteins in Gels
  • Unit 7.5 Overview of Digital Electrophoresis Analysis
• Chapter 8 Blotting
  • Unit 8.1 Overview of Blotting
  • Unit 8.2 Nucleic Acid Blotting: Southern and Northern
  • Unit 8.3 Protein Blotting: Immunoblotting
  • Unit 8.4 Labeling DNA and Preparing Probes
• Chapter 9 Microscopy
  • Unit 9.1 Conventional Light Microscopy
  • Unit 9.2 Immunofluorescence Microscopy
  • Unit 9.3 In Situ Hybridization: Fruit Fly Embryos and Tissues
• Chapter 10 Enzymatic Reactions
  • Unit 10.1 Working with Enzymes
  • Unit 10.2 Overview of PCR
  • Unit 10.3 Real‐Time PCR
• Chapter 11 Bioinformatics
  • Unit 11.1 Using NCBI BLAST
  • Unit 11.2 Submitting a Sequence to GenBank
  • Unit 11.3 Beyond Simple Homology Searches: Multiple Sequence Alignments and Phylogenetic Trees
  • Unit 11.4 Using Model Organism Databases (MODs)
• Chapter 12 Outsourcing Guides
  • Unit 12.1 DNA Sequencing: An Outsourcing Guide
  • Unit 12.2 Mass Spectrometry: An Outsourcing Guide
• Chapter 13 Manipulating DNA and Gene Expression
  • Unit 13.1 LOV Domains as In Vivo Fluorescent Reporters of Protein Expression
  • Unit 13.2 Expression of Recombinant Genes in the Yeast Pichia pastoris
• Appendix 1 Safety
  • Appendix 1A General Laboratory Safety and Working with Hazardous Chemicals
  • Appendix 1B Risk Assessment in the Research Laboratory
• Appendix 2 Experiment Documentation and Data Storage
  • Appendix 2A Laboratory Notebooks and Data Storage
• Appendix 3 Considerations When Altering Digital Images
  • Appendix 3A Ethical Considerations When Altering Digital Images
  • Appendix 3B Practical Considerations When Altering Digital Images
  • Appendix 3C Digital Image Processing and Analysis with ImageJ
• Appendix 4 Data Analysis
  • Appendix 4A Statistical Analysis
  • Appendix 4B Basics of Statistical Analysis
• Appendix 5 Getting Your Data Out Into the World
  • Appendix 5A Preparing and Presenting a Poster
  • Appendix 5B Preparing and Presenting a Talk
  • Appendix 5C How to Write a Research Manuscript
  • Appendix 5D Bibliographies Made Easy: A Guide to Using Citation Software to Create Bibliographies

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