Current Protocols in Chemical Biology

Table of Contents

•   Volume 5
  •   Issue 1
    •   Advances in Lectin Microarray Technology: Optimized Protocols for Piezoelectric Print Conditions
    •   In Vivo Imaging and Biochemical Characterization of Protease Function Using Fluorescent Activity‐Based Probes
    •   Bioorthogonal Profiling of Protein Methylation (BPPM) Using an Azido Analog of S‐Adenosyl‐L‐Methionine
    •   Profiling Protein Methylation with Cofactor Analog Containing Terminal Alkyne Functionality
•   Volume 4
  •   Issue 1
    •   Gene Assembly from Chip‐Synthesized Oligonucleotides
    •   Labeling Substrate Proteins of Poly(ADP‐ribose) Polymerases with Clickable NAD Analog
    •   On‐Bead Two‐Color (OBTC) Cell Screen for Direct Identification of Highly Selective Cell Surface Receptor Ligands
    •   High‐Throughput RT‐PCR for Small‐Molecule Screening Assays
    •   Measurement of Protein Phosphorylation Stoichiometry by SRM‐MS
    •   Kinase‐Catalyzed Biotinylation of Peptides, Proteins, and Lysates
  •   Issue 2
    •   Bioorthogonal Chemical Reporters for Analyzing Protein Sulfenylation in Cells
    •   Nanostructure‐Initiator Mass Spectrometry (NIMS) for the Analysis of Enzyme Activities
    •   In Situ Hybridization Assay‐Based Small‐Molecule Screening in Zebrafish
    •   High‐Throughput Phosphoproteomics from Formalin‐Fixed, Paraffin‐Embedded Tissues
  •   Issue 3
    •   Selecting, Acquiring, and Using Small Molecule Libraries for High‐Throughput Screening
    •   Dealing with the Data Deluge: Handling the Multitude of Chemical Biology Data Sources
    •   Functional Assays on High‐Content Protein Microarrays
    •   One‐Pot Multienzyme Synthesis of Lewis x and Sialyl Lewis x Antigens
    •   Metallomics Using Inductively Coupled Plasma Mass Spectrometry
  •   Issue 4
    •   SHAPE–Seq: High‐Throughput RNA Structure Analysis
    •   In Vivo Rapid Assessment of Compound Exposure (RACE) for Profiling the Pharmacokinetics of Novel Chemical Probes
    •   A High‐Throughput Screening Method for Identification of Inhibitors of the Deubiquitinating Enzyme USP14
    •   Specificity Profiling of Protein‐Binding Domains Using One‐Bead‐One‐Compound Peptide Libraries
    •   A Screening Protocol for Identification of Functional Mutants of RNA Editing Adenosine Deaminases
•   Volume 3
  •   Issue 1
    •   Parallel High‐Throughput Automated Assays to Measure Cell Growth and Beta‐Galactosidase Reporter Gene Expression in the Yeast Saccharomyces cerevisiae
    •   Development of Chemical Probes for Biochemical Detection and Cellular Imaging of Myristoylated and Palmitoylated Proteins
    •   Imaging of Endogenous RNA Using Genetically Encoded Probes
    •   Development of In‐Cell Western Assays Using Far‐Red Fluorophores
  •   Issue 2
    •   Miniaturized High‐Throughput Fluorescent Assay for Conversion of NAD(P)H to NAD(P)
    •   Derivatization of Free Natural Glycans for Incorporation onto Glycan Arrays: Derivatizing Glycans on the Microscale for Microarray and Other Applications
    •   Visualization and Identification of Fatty Acylated Proteins Using Chemical Reporters
  •   Issue 3
    •   Chemical Synthesis of Hydrocarbon‐Stapled Peptides for Protein Interaction Research and Therapeutic Targeting
    •   Triple‐Addition Assay Protocols for Detecting and Characterizing Modulators of Seven‐Transmembrane Receptors
    •   Compound Management: Guidelines for Compound Storage, Provision, and Quality Control
  •   Issue 4
    •   Copper‐Catalyzed Azide–Alkyne Click Chemistry for Bioconjugation
    •   Target Identification Using Drug Affinity Responsive Target Stability (DARTS)
    •   Analyzing In Vivo Metabolite‐Protein Interactions by Large‐Scale Systematic Analyses
•   Volume 2
  •   Issue 1
    •   Cross‐Coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base‐Modified DNA
    •   Chemical Genetic Approach for Kinase‐Substrate Mapping by Covalent Capture of Thiophosphopeptides and Analysis by Mass Spectrometry
    •   Construction and Use of Glycan Microarrays
  •   Issue 2
    •   Directed Evolution of DNA Polymerases: Construction and Screening of DNA Polymerase Mutant Libraries
    •   Nucleotide Analogues as Probes for DNA and RNA Polymerases
    •   Quantitative Glycomics of Cultured Cells Using Isotopic Detection of Aminosugars with Glutamine (IDAWG)
    •   Targeted Degradation of Proteins by PROTACs
    •   Site‐Specific Protein Bioconjugation via a Pyridoxal 5′‐Phosphate‐Mediated N‐Terminal Transamination Reaction
  •   Issue 3
    •   Small‐Molecule Library Synthesis on Silicon‐Functionalized SynPhase Lanterns
    •   High‐Throughput Assessment of Mammalian Cell Viability by Determination of Adenosine Triphosphate Levels
    •   Preparation, Characterization, and Application of Optical Switch Probes
    •   Experimental Design Considerations for In Vitro Non‐Natural Glycan Display via Metabolic Oligosaccharide Engineering
  •   Issue 4
    •   High‐Throughput Assessment of Bacterial Growth Inhibition by Optical Density Measurements
    •   Supported Membrane Formation, Characterization, Functionalization, and Patterning for Application in Biological Science and Technology
    •   Mass Spectrometry‐Based Identification of Protein Kinase Substrates Utilizing Engineered Kinases and Thiophosphate Labeling
•   Volume 1
  •   Issue 1
    •   Fluorescence Polarization (FP) Assays for Monitoring Peptide‐Protein or Nucleic Acid‐Protein Binding
    •   Spatiotemporal Dynamics of Kinase Signaling Visualized by Targeted Reporters
    •   Activity‐Based Protein Profiling (ABPP) and Click Chemistry (CC)–ABPP by MudPIT Mass Spectrometry
    •   Overview of Liquid Handling Instrumentation for High‐Throughput Screening Applications
    •   Target Identification by Diazirine Photo‐Cross‐Linking and Click Chemistry

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