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Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

Last Update: October 02, 2017
Print ISSN: 1934-3639
Online ISSN: 1934-3647


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What's New in Current Protocols in Molecular Biology
Supplement 120, October 2017

Unit 4.29 A Detailed Protocol for Subcellular RNA Sequencing (subRNA-seq)
Abstract | Full Text: HTML  PDF

Unit 21.34 Single-Assay Profiling of Nucleosome Occupancy and Chromatin Accessibility
Abstract | Full Text: HTML  PDF

Unit 21.35 Transposon Insertion Site Sequencing (TIS-Seq): An Efficient and High-Throughput Method for Determining Transposon Insertion Site(s) and Their Relative Abundances in a PiggyBac Transposon Mutant Pool by Next-Generation Sequencing
Abstract | Full Text: HTML  PDF

Unit 31.10 Production of Purified CasRNPs for Efficacious Genome Editing
Abstract | Full Text: HTML  PDF

Unit 31.11 CASAAV: A CRISPR-Based Platform for Rapid Dissection of Gene Function In Vivo
Abstract | Full Text: HTML  PDF

An essential tool for anyone at the forefront of molecular biology research, Current Protocols in Molecular Biology—the first Current Protocols title—remains the benchmark by which all other protocol resources are judged. With an extensive range of information, from basic methods to advanced procedures, Current Protocols in Molecular Biology provides incomparable coverage of this ever-expanding field.

A subscription gives you access to all the content in the collection plus four quarterly issues of new and updated content. Current Protocols in Molecular Biology...

  • covers basic methods, such as nucleic acid isolation, purification, and quantition
  • contains updated information and protocols on rapidly changing areas such as Next-Generation Sequencing, RNAi, and zincfinger nucleases
  • offers advanced procedures for microarray analysis, chromatin assembly and analysis, single-cell analysis and gene silencing, among others
  • explores specialized areas, such as metabolomics

Edited by: Fred M. Ausubel (Massachusetts General Hospital and Harvard Medical School); Roger Brent (Fred Hutchinson Cancer Research Center); Robert E. Kingston (Massachusetts General Hospital and Harvard Medical School); David D. Moore (Baylor College of Medicine); J.G. Seidman (Harvard Medical School); Kevin Struhl (Harvard Medical School). Past Editor: John A. Smith (University of Alabama at Birmingham); Guest Editors: Donald M. Coen (Harvard Medical School); Andrew F. Gardner (New England Biolabs); Ruslan I. Sadreyev (Massachusetts General Hospital and Harvard Medical School); Barton E. Slatko (New England Biolabs). Past Guest Editors: Lisa M. Albright (Austin, Texas); Mark L. Borowsky (Massachusetts General Hospital); Reuben Shaw (The Salk Institute for Biological Studies); Carolyn L. Smith (Baylor College of Medicine); Ajit Varki (University of California San Diego); Mary C. Wildermuth (University of California Berkeley).

Developmental Editor: Gwen P. Taylor

While the authors, editors, and publisher believe that the specification and usage of reagents, equipment, and devices, as set forth in this book, are in accord with current recommendations and practice at the time of publication, they accept no legal responsibility for any errors or omissions, and make no warranty, express or implied, with respect to material contained herein. In view of ongoing research, equipment modifications, changes in governmental regulations, and the constant flow of information relating to the use of experimental reagents, equipment, and devices, the reader is urged to review and evaluate the information provided in the package insert or instructions for each chemical, piece of equipment, reagent, or device for, among other things, any changes in the instructions or indication of usage and for added warnings and precautions. This is particularly important in regard to new or infrequently employed chemicals or experimental reagents. Moreover, the information presented herein is not a substitute for professional judgment, especially as concerns any applications in a clinical setting or the interpretation of results thereby obtained.