Ultra-Rapid and Ultra-Sensitive Detection of Proteins in Chemiluminescent Western Blotting

The UVP BioSpectrum® Imaging System for high sensitivity detection of proteins in conjunction with the FemtoMax™ chemiluminescent-HRP substrate provides for extremely fast and sensitive detection of proteins by Western blot. We demonstrate the detection of 6 different commonly used epitope tags using Rockland Immunochemicals’s epitope tag specific antibodies and a new 12-epitope tag control marker. The combination of high quality primary and secondary antibodies coupled with the new FemtoMax™ HRP substrate and the BioSpectrum® Imaging System allows the detection of femtogram amounts of target proteins with excellent sensitivity and specificity.