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Current Protocols Videos

 

  • Calibration of the needle with masses

  • Chromosomal stretching experiment using a Xenopus in vitro-assembled mitotic chromosome (Introduction/Basic Protocol)

  • Coating grids with Formvar

  • Counting and Determining the Viability of Cultured Cells

  • Fixation of cells for cryo-immunogold labeling

  • Freezing, Thawing, and Packaging Cells for Transport

  • Labeling and washing grid-mounted sections

  • Methylcellulose embedding

  • Picking up sections after ultrathin sectioning

  • Plunge freezing

  • Preparing a mounted eyelash for handling sections

  • Sucrose infiltration tissues/cells fixed for cryo-immunogold labeling

  • Trypsinizing and Subculturing Mammalian Cells

  • Tubule formation on a vesicle during endocytosis

  • Ultrathin cryo-sectioning

  • Ultrathin section retrieval and transfer to Formvar-coated grids

  • Preparation and fractionation of xenopus laevis egg extracts
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