Pool worms and wash them in PBS by centrifugation, then rapidly freeze them in liquid N2. Grind them over a dry ice-ethanol bath using a mortar and pestle and add cold homogenization buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM DTT, 1% Triton X-100, protease inhibitors just before use). You can then clear the lysate by centrifuging at 15,000 × g for 20 minutes at 4°C. Alternatively, you can pool and wash worms as above, then resuspend worms in cold homogenization buffer and lyse by sonication on ice. Finally, instead of sonicating you can pass the resuspended worms through a French press (1200 Kg/cm2) and then clear the lysate as above.
Pool worms and wash them in PBS by centrifugation, then rapidly freeze them in liquid N2. Grind them over a dry ice-ethanol bath using a mortar and pestle and add cold homogenization buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM DTT, 1% Triton X-100, protease inhibitors just before use). You can then clear the lysate by centrifuging at 15,000 × g for 20 minutes at 4°C.
Alternatively, you can pool and wash worms as above, then resuspend worms in cold homogenization buffer and lyse by sonication on ice. Finally, instead of sonicating you can pass the resuspended worms through a French press (1200 Kg/cm2) and then clear the lysate as above.