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DGH
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Joined: Mar 12 2009
User offline. Last seen 2 weeks 5 days ago.

Hello Current Protocols online users: We are very pleased to announce the launching of the new website. It has been in the planning stages for many months now and we are happy with the results. However, as with any website launch, there are bound to be issues that we, and you, will come across. Please feel free to alert us of any bugs, functionality issues, typos, or suggestions--we are always interested in hearing what our community has to say. Looking forward to growing with you!

CP Online Team

Anonymous
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Protocol missing

Hi,

There was a protocol on your old website called "Whole Blood Processing for Measurement of Signaling Proteins by Flow Cytometry", unit 9.27. I can't find it now. Could someone explain why?

Thank you,

Lily

Anonymous
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nucleic acid synthesis

can bacteria synthesize their own dna authonomously if so how apart from trasformation trasduction transfection or even conjugation

vbc
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Joined: Mar 4 2009
User is online
protocol missing

Hi Lily, CP Cytometry Unit 9.27, "Whole Blood Processing for Measurement of Signaling Proteins by Flow Cytometry," is available on the site now.

Tom Downey
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Joined: May 5 2009
User offline. Last seen 2 years 32 weeks ago.
nucleic acid synthesis

Just like other organisms, bacteria synthesize their DNA from existing templates during the process of replication. There are a couple of sites that explain this well. There is a really nice flash animation showing  the replication process at http://www.wiley.com/college/pratt/0471393878/student/animations/dna_rep.... Wikipedia has a good overview of replication with some specifics about bacterial replication at http://en.wikipedia.org/wiki/DNA_replication.

Jack Chen
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Joined: Jun 1 2009
User offline. Last seen 2 years 35 weeks ago.
A new chapter -- OrthoCluster for synteny blocks

The identification of synteny blocks, both perfect synteny blocks and ones that allow minor interruptions, among multiple genomes is a challenging problem. We have recently developed a new program OrthoCluter to address this problem. A website has bene set up (http://genome.sfu.ca/orthoclusterdb/) for online access. A new chapter has been written to demonstrate how to run it online and locally. The chapter is being published.

Jack Chen

Anonymous
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GPT protocol

Dear any one can suggest GPT protol for bacterial & yest mould Limit.we are going to test herbal powder.

 

Amanda Grey
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Joined: Dec 4 2009
User offline. Last seen 2 years 9 weeks ago.
Yes. I guess thay have the

Yes. I guess thay have the ability to synthesize their own dna authonomously :)

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