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How to Write a Research Manuscript

Deborah J. Frank¹

¹Washington University, St. Louis, Missouri

Publication Name:

Current Protocols Essential Laboratory Techniques

Unit Number:

Appendix 5C

DOI:

10.1002/9780470089941.eta05cs02

Online Posting Date:

December, 2009

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Abstract

This unit provides a step-by-step guide to help you turn your high-quality data into a high-quality manuscript for publication in a scientific journal. It covers all aspects of the writing process, including: choosing a journal to which to submit your paper, how to proceed through writing each section, formulating your “story,” making figures, soliciting constructive criticism, and navigating the review process. Curr. Protoc. Essential Lab. Tech. 2:A.5C.1-A.5C.18. © 2009 by John Wiley & Sons, Inc.

Keywords: manuscript; journal; figure; review; publication

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Introduction
Examples of Good Papers to Model
Deciding When to Start Writing the Manuscript
Before You Start Writing
Getting Started on the Actual Writing
Putting it All Together
Soliciting and Responding to Constructive Criticisms
Three Style Issues
Cover Letter
Submission
The Review Process
Rebuttal Letter
After Your Paper is Accepted
Conclusion
Literature Cited
Figures

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Figures

Figure A.5C.1

Myosin VI molecules used in this study. (A) Schematic diagram of constructs. GFP, green fluorescent protein; Head, motor domain; 1, 2, inserts found uniquely in class VI myosins; IQ, IQ motif; P-Tail, proximal tail; CC, core coiled coil; G-Tail, globular tail; GCN4, leucine zipper dimerization domain. Drawings are not to scale. (B) Western blots of testis extracts from flies expressing the indicated myosin VI molecules. The top halves of the blots were probed with polyclonal anti-myosin VI antibody and the bottom halves with anti-tubulin antibody. 1×, 2×, and 4× indicate the number of copies of the indicated transgene. Except for wild type, all on the blot on the left are in a myosin VI mutant background. Those on the blot on the right are in wild-type background, so both endogenous and exogenous myosin VI are evident. Sizes are indicated in kDa.

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Figure A.5C.2

Range of actin displacement phenotypes observed in the capping protein, arpc1 double mutant bristles. Each image is a projection of optical sections. Insets are computed cross-sections. Scale bar does not apply to insets.

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Literature Cited

Literature Cited
	Dyson, S. and Gurdon, J.B. 1998. The interpretation of position in a morphogen gradient as revealed by occupancy of activin receptors. Cell 93:557-568.
	Freeman, M. 1996. Reiterative use of the EGF receptor triggers differentiation of all cell types in the Drosophila eye. Cell 87:651-660.
	Noller, H.F., Hoffarth, V., and Zimniak, L. 1992. Unusual resistance of peptidyl transferase to protein extraction procedures. Science 256:1416-1419.