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Parenteral Injections
Publication Name:
Current Protocols in Immunology
Unit Number:
Unit 1.6
DOI:
10.1002/0471142735.im0106s73
Online Posting Date:
July, 2006 Abstract
This unit describes the techniques for the following routes of injection for mice, rats, hamsters and rabbits: intramuscular, intradermal, subcutaneous, intravenous, intraperitoneal, footpad, and intrathymic. Guidelines are also given regarding injection volumes and temperatures, and the use of proper restraints.
Table of Contents
- Section II: Use of Mouse, Rat, Hamster, and Rabbit
- Unit Introduction
- Basic Protocol 1: Intramuscular Injection of Mouse, Rat, Hamster, and Rabbit
- Basic Protocol 2: Intradermal Injection of Mouse, Rat, Hamster, and Rabbit
- Basic Protocol 3: Subcutaneous Injection of Mouse, Rat, Hamster, and Rabbit
- Basic Protocol 4: Intravenous Injection of the Mouse
- Basic Protocol 5: Intravenous Injection of the Rat
- Basic Protocol 6: Intravenous Injection of the Hamster
- Basic Protocol 7: Intravenous Injection of the Rabbit
- Basic Protocol 8: Intraperitoneal Injection of Mouse, Rat, Hamster, and Rabbit
- Basic Protocol 9: Footpad Injection of the Mouse
- Basic Protocol 10: Intrathymic Injection of the Mouse
- Bibliography
- Figures
- Tables
Materials
Basic Protocol 1: Intramuscular Injection of Mouse, Rat, Hamster, and Rabbit
Materials
- Injectate
- 1- to 3-ml syringe with 22- to 30-G needle
Basic Protocol 2: Intradermal Injection of Mouse, Rat, Hamster, and Rabbit
Materials
- 70% ethanol
- Injectate
- Clippers with #40 blade
- Gauze sponge or swab
- 1- to 3-ml syringe with 25- to 30-G needle
- Additional reagents and equipment for handling and restraint (unit
Basic Protocol 3: Subcutaneous Injection of Mouse, Rat, Hamster, and Rabbit
Materials
- Injectate
- 1- to 3-ml syringe with 22- to 30-G needle
Basic Protocol 4: Intravenous Injection of the Mouse
Materials
- Injectate
- 70% ethanol
- 1-ml syringe with 25- to 30-G needle
- Restrainer (unit
- Heat lamp or beaker containing warm water
- Gauze sponge or swab
Basic Protocol 5: Intravenous Injection of the Rat
Materials
- Injectate
- 1-ml syringe with 25- to 30-G needle
- Additional reagents and equipment for anesthesia (unit
Basic Protocol 6: Intravenous Injection of the Hamster
Materials
- 70% ethanol
- Injectate
- Clippers with #40 blade
- Gauze sponge or swab
- 1-ml syringe with 25- to 30-G needle
- Additional reagents and equipment for anesthesia (unit
Basic Protocol 7: Intravenous Injection of the Rabbit
Materials
- 70% ethanol
- Injectate
- Restrainer (unit
- Gauze sponge or swab
- 1- or 3-ml syringe with 25- to 30-G butterfly infusion set
Basic Protocol 8: Intraperitoneal Injection of Mouse, Rat, Hamster, and Rabbit
Materials
- Injectate
- 1- or 3-ml syringe with 22- to 25-G needle (mouse, rat, and hamster) or 18- to 23-G, 1-in. needle (rabbit)
Basic Protocol 9: Footpad Injection of the Mouse
Materials
- Injectate
- 1-ml syringe with 25-G needle
Basic Protocol 10: Intrathymic Injection of the Mouse
Materials
- 70% ethanol
- Injectate
- Surgical board
- Gauze sponge or swab
- Scalpel handle (#3) and scalpel blade (#15)
- Thumb forceps
- Iris scissors, straight
- 1-ml syringe with 28- to 30-G needle
- Wound clips or 4-0 suture material with needle
- Additional reagents and equipment for anesthesia (unit
Looking for materials? Suppliers Guide
Figures
-
Figure 1.6.1Intramuscular injection of the mouse. -
Figure 1.6.2Intramuscular injection of the rabbit. -
Figure 1.6.3Subcutaneous injection of the mouse. -
Figure 1.6.4Intravenous injection of the mouse in the tail vein. -
Figure 1.6.5Intravenous injection of the rat in the dorsal penile vein. -
Figure 1.6.6Intravenous injection of the hamster in the anterior cephalic vein. -
Figure 1.6.7Intravenous injection of the rabbit. With the rabbit in a restrainer, apply pressure over the vein at the base of the ear and introduce the needle of the infusion set into the engorged marginal ear vein. -
Figure 1.6.8Intraperitoneal injection of the mouse. -
Figure 1.6.9Intrathymic injection of the mouse.
Literature Cited
| Literature Cited | |
| Tuffery, A.A. 1987. Laboratory Animals: An Introduction for New Experimenters, pp. 225-226. John Wiley & Sons, Chichester, England. | |
| Waynforth, H.B. 1980. Experimental and Surgical Technique in the Rat, p. 39. Academic Press, Orlando, Fla. | |
| Key References | |
| Fox, J.G., Anderson, L.C., Loew, F.M., and Quimby, F.W. (eds.). 2002. Laboratory Animal Medicine, 2nd ed. Academic Press, Orlando, Fla. | |
| Provides additional details for injection methods. | |
| Goldschneider, I., Komschlies, K.L., and Greiner, D.L. 1986. Studies of thymocytes in rats and mice. J. Exp. Med. 163:1-17. | |
| Provides detailed description of the intrathymic injection technique. | |
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