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Removal of Lymphoid Organs

J.P. Reeves¹, P.A. Reeves¹

¹Food and Drug Administration, Bethesda, Maryland

Publication Name:

Current Protocols in Immunology

Unit Number:

Unit 1.9

DOI:

10.1002/0471142735.im0109s01

Online Posting Date:

May, 2001

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Abstract

Although for most experiments lymphoid tissue is removed from the freshly sacrificed animal, there are several circumstances which require the surgical removal, under anesthesia, of either the thymus or spleen, and the recovery of the animal for further investigation. This unit describes survival surgery for removal of the adult spleen as well as the adult and neonatal thymus of the mouse.

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Unit Introduction
Basic Protocol: Removal of Mouse Lymphoid Organs
Key References
Figures

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Materials

Basic Protocol: Removal of Mouse Lymphoid Organs

Materials

70% ethanol
Hanks balanced salt solution (HBSS; appendix 2A) or tissue culture medium

Iris scissors (straight or angled)
Iris forceps (curved)
Tissue culture plate

Additional reagents and equipment for euthanasia (unit 1.8)

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Figures

Figure 1.9.1

Midline skin incision for removal of lymphoid organs.

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Figure 1.9.2

The lymph nodes and spleen of the mouse.

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Literature Cited

Key References
	Sjodin, K., Dalmasso, A.P., Smith, J.M., and Martinez, C. 1963. Thymectomy in newborn and adult mice. Transplantation 1:521-525.
	Van Alten, P.J. 1984. Thymectomy and engraftment of thymic tissue. Methods Enzymol. 108:10-19.