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Surgical Removal of Mouse Salivary Glands

Stipan Jonjic¹

¹University of Rijeka, Rijeka, Croatia, Costa Rica

Publication Name:

Current Protocols in Immunology

Unit Number:

Unit 1.11

DOI:

10.1002/0471142735.im0111s43

Online Posting Date:

August, 2001

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Abstract

Surgical removal of the salivary glands is a simple procedure aimed at providing glandular tissues for studies in histopathology, immunohistology, DNA and RNA analysis, cytokine production, and virus detection and isolation. This unit describes the surgical removal of the salivary glands in mice, but a similar protocol is applicable to rats and other rodents.

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Unit Introduction
Basic Protocol
Commentary
Literature Cited
Figures

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Materials

Basic Protocol

Materials

>3-week-old mouse
70% ethanol
Complete RPMI-1640, 4°C (appendix 2A)

Operating board
Tooth holder: surgical suture or rubber band and dissecting needles
Rolled-up gauze pad
Dissection needles
Iris forceps with straight or curved ends
Iris scissors (straight or curved)
Tissue culture plate, on ice

Additional reagents and equipment for mouse euthanasia (unit 1.8)

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Figures

Figure 1.11.1

Surgical removal of salivary glands. (A) Positions of major salivary glands after dissection of the surrounding connective tissue and superficial cervical lymph nodes. (B) Removal of both submandibular and major sublingual glands. (C) Removal of the right parotid gland.

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Literature Cited

Literature Cited
	Facini, J.M., Abbot, D.P., and Paulus, G.J.J. (eds.) 1990. Digestive system. In Mouse Histopathology. A Glossary for Use in Toxicity and Carcinogenicity Studies, pp. 72-74. Elsevier, Amsterdam.
	Jonjic, S., Mutter, W., Weiland, F., Reddehase, M.J., and Koszinowski, U.H. 1989. Site-directed persistent cytomegalovirus infection after selective long-term depletion of CD4-positive T lymphocytes. J. Exp. Med. 169:1199-1212.
	McQuone, S.J. 1999. Acute viral and bacterial infections of the salivary glands. Otolaryngol. Clin. N. Am. 32:793-811.
	Mocarski, E.S. 1996. Cytomegaloviruses and their replication. In Fields Virology (B.N. Fields, D.M. Knipe, and P.M. Howley, eds.) pp. 2447-2492. Lippincott-Raven, Philadelphia.
	Osborn, J.E. and Walker, D.L. 1971. Virulence and attenuation of murine cytomegalovirus. Infect. Immun. 3:228-236.
	Polic, B., Hengel, H., Krmpotic, A., Trgovcich, J., Pavic, I., Lucin, P., Jonjic, S., and Koszinowski, U.H. 1998. Hierarchical and redundant lymphocyte subset control precludes cytomegalovirus replication during latent infection. J. Exp. Med. 188:1047-1054.