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Techniques in Aseptic Rodent Surgery
Publication Name:
Current Protocols in Immunology
Unit Number:
Unit 1.12
DOI:
10.1002/0471142735.im0112s82
Online Posting Date:
August, 2008 Abstract
Performing aseptic survival surgery in rodents can be challenging. This unit describes some basic principles to assist clinicians, researchers, and technicians in becoming proficient in performing aseptic rodent surgery. Curr. Protoc. Immunol. 82:1.12.1-1.12.14. © 2008 by John Wiley & Sons, Inc.
Keywords: aseptic surgery; technique; rodent; surgical preparation; instrument preparation; suture material; anesthesia; analgesia; surgical gloves
Table of Contents
- Introduction
- Strategic Planning
- Basic Protocol 1: Surgical Preparation of the Animal
- Performing Aseptic Surgery
- Basic Protocol 2: Performing Aseptic Surgery with Sterile Surgical Gloves
- Alternate Protocol: Performing Aseptic Surgery with Clean Exam Gloves
- Commentary
- Literature Cited
- Figures
Materials
Basic Protocol 1: Surgical Preparation of the Animal
Materials
- Animal
- Iodophor or chlorhexidine scrub
- 70% alcohol
- Mini-clipper with no. 0000 blade
- Adhesive tape (1- to 2-in. wide)
- 2 × 2–in. gauze pads
- Cotton-tipped applicators
Basic Protocol 2: Performing Aseptic Surgery with Sterile Surgical Gloves
Materials
- Anesthetized and surgically prepped animal
- 70% ethanol
- Surgical attire including:
- Cap
- Mask
- Laboratory coat
- Sterile surgical instruments and equipment
- Warming device (see Strategic Planning)
- Sterile surgical gloves
- Surgical drape
- Animal cage
- Instrument sterilizer
Alternate Protocol: Performing Aseptic Surgery with Clean Exam Gloves
Additional Materials (also see Basic Protocol 2 )
- Clean exam gloves (not sterile surgical gloves)
Looking for materials? Suppliers Guide
Figures
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Figure 1.12.1(A) Good surgical site and (B) poor surgical site. -
Figure 1.12.2Anesthesia machine with precision calibrated vaporizer. -
Figure 1.12.3Downdraft table. -
Figure 1.12.4Chemical fume hood. -
Figure 1.12.5Type IIB biosafety cabinet. -
Figure 1.12.6Charcoal canister. -
Figure 1.12.7Simple peel pack. -
Figure 1.12.8Complex surgical pack. -
Figure 1.12.9Hot-bead sterilizer. -
Figure 1.12.10Flash dry-heat sterilizer. -
Figure 1.12.11Shows the organized surgical instruments. -
Figure 1.12.12Between surgeries, the tips of the instruments should be covered. -
Figure 1.12.13Paper surgical drape. -
Figure 1.12.14Plastic adhesive surgical drape. -
Figure 1.12.15Sterile gauze pads used for surgical drapes. -
Figure 1.12.16Sterile tips of the instruments are placed on a sterile field. -
Figure 1.12.17Arrows indicate space between drape and instruments that is not sterile. -
Figure 1.12.18A wet area on a paper or cloth drape acts as a wick to pull bacteria through from the nonsterile surface below.
Videos
Literature Cited
| Literature Cited | |
| Flecknell, P.A. 1996. Laboratory Animal Anaesthesia, 2nd ed. Academic Press, London. | |
| Knecht, C.D., Algernon, R.A., Williams, D.J., and Johnson, J.H. 1987. Fundamental Techniques in Veterinary Surgery. WB Saunders Company, Philadelphia, Pennsylvania. | |
| Kohn, D.H., Wixson, S.K., White, W.J., and Benson G.J. 1997. Anesthesia and Analgesia in Laboratory Animals. Academic Press, San Diego, California. | |
| National Institutes of Health Animal Research Advisory Committee (NIH ARAC). 2005. Guidelines for Survival Rodent Surgery. (http://oacu.od.nih.gov/ARAC/surguide.pdf) | |
| National Research Council (NRC). 1996. "Veterinary Care". In Guide for the Care and Use of Laboratory Animals (Guide), pp. 556-570. National Academy Press, Washington, DC. (http://books.nap.edu/readingroom/books/labrats/) | |
| Swindle, M.M., Vogler, G.A., Fulton, L.K., Marini, R.P., and Popilskis, S. 2002. "Preanesthesia, anesthesia, analgesia, and euthanasia". In Laboratory Animal Medicine (J.G. Fox, L.C. Anderson, F.M. Loew, and F.W. Quimby, eds.) pp. 955-966. Academic Press, San Diego, California. | |
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