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cDNA Libraries

David D. Moore¹

¹Massachusetts General Hospital And Harvard Medical School, Boston, Massachusetts

Publication Name:

Current Protocols in Molecular Biology

Unit Number:

Unit 5.2

DOI:

10.1002/0471142727.mb0502s01

Online Posting Date:

May, 2001

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Abstract

This unit provides a basic description of issues that require careful consideration before undertaking construction of cDNA libraries. Topics include: generating a double-stranded DNA copy of the mRNA, the quality of the mRNA, the relative abundance of the clone of interest, the choice of screening method, the size of the library that will be necessary to include the clone of interest, and choices of linkers or adaptors for insertion into the vector.

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Unit Introduction
Key References

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Literature Cited

Key References
	Frischauf, A.-M., Lehrach, H., Poustka, A., and Murray, N. 1983. Lambda replacement vectors carrying polylinker sequences. J. Mol. Biol. 170:827.
	Huynh, T., Young, R., and Davis, R. 1984. Construction and screening cDNA libraries in gt10 and gt11. In DNA Cloning, Vol. 1: A Practical Approach (D. Glover, ed.) pp. 49-78. IRL Press, Oxford.