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Overview of Image Analysis, Image Importing, and Image Processing using Freeware

E.L. Bearer¹

¹Brown University, Providence, Rhode Island

Publication Name:

Current Protocols in Molecular Biology

Unit Number:

Unit 14.15

DOI:

10.1002/0471142727.mb1415s63

Online Posting Date:

August, 2003

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Abstract

Quantitative image analysis turns microscopic data that might otherwise be merely descriptive into reliable mechanistic information. This unit provides an overview of the types of analysis that have been useful in the past, as well as descriptions of emerging applications. In addition, two protocols are included for importing data and for the first steps of data manipulation in the more common analytical applications of the freeware, NIH Image and ImageJ, as well as commercially available imaging software, Adobe Photoshop.

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Unit Introduction
Basic Protocol: Image Processing Using NIH Image 1.62
Alternate Protocol: Image Processing Using ImageJ
Commentary
Literature Cited

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Materials

Basic Protocol: Image Processing Using NIH Image 1.62

Materials

Color-capable Macintosh computer with ³2 Mb of free RAM and the following programs:
- MacOS v. 7 or later, or OS X capable of running programs in Classic mode
- Web browser, e.g., Netscape or MS Internet Explorer
- Stuffit Expander
- Adobe Photoshop
Digital images and image sequences: these can be translated from a wide variety of file types, although TIFF formats are easiest to work with; importing from many other document types is handled through an Input-Output Macro that downloads with NIH Image

Alternate Protocol: Image Processing Using ImageJ

Materials

Color-capable Macintosh computer or PC with 64 Mb (256 Mb recommended) of free RAM and the following programs:
- MacOS v. 7.6 or higher or Windows 95 or higher with Java Runtime Environment 1.1.3
- MRJ 2.1 (download from http://www.apple.com/java/)
- QuickTime for Java (installed with Mac OS 9.04 or higher, or can be installed from QuickTime 1 “custom” Install menu)

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Literature Cited

Literature Cited
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