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Receptor Binding Techniques

Kirk A. Frey¹, Roger L. Albin²

¹University of Michigan, Ann Arbor, Michigan
²University of Michigan and Ann Arbor Veterans' Administration Medical Center, Ann Arbor, Michigan

Publication Name:

Current Protocols in Neuroscience

Unit Number:

Unit 1.4

DOI:

10.1002/0471142301.ns0104s00

Online Posting Date:

May, 2001

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Abstract

This overview first discusses issues relating to the selection of radioligand for receptor binding assays, including the isotopic label and considerations pertaining to the pharmacological and chemical profile of the ligand. This is followed by a section on characterization of ligand-binding assays, starting with tissue preparation methods, followed by detection of specific binding, determination of incubation and washing conditions and a discussion of saturation and competition assay formats. Quantification of the assay results can be accomplished by autoradiography or film densitometry. Finally, methods and considerations for analysis of the resulting data are presented.

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Unit Introduction
Radioligand Selection
Characterization of Ligand-Binding Assays
Quantification of Ligand Binding
Analysis of Autoradiographic Data
Conclusion
Literature Cited
Tables

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Literature Cited

Literature Cited
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