User Ratings

Easy to Follow Achieved Expected Results Overall Rating Add your comments

Electronic Imaging in Neuroscience

Kenneth R. Spring¹

¹National Institutes of Health National Heart, Lung and Blood Institute, Bethesda, Maryland

Publication Name:

Current Protocols in Neuroscience

Unit Number:

Unit 2.4

DOI:

10.1002/0471142301.ns0204s18

Online Posting Date:

May, 2002

GO TO THE FULL TEXT:

PDF or HTML at Wiley Online Library

Are you the author of this protocol? Login or register and return to this page.

Abstract

This unit is intended to aid the neuroscientist in understanding the basics of image detectors and selecting a suitable camera for various neuroscience research applications. A procedure is described for evaluating cameras in the laboratory.

GO TO THE FULL PROTOCOL:

PDF or HTML at Wiley Online Library

Unit Introduction
Camera Basics
Low-Light-Level Imaging of Fluorescence
Choosing the Appropriate Camera
Evaluating Camera Performance
Bibliography
Figures

GO TO THE FULL PROTOCOL:

PDF or HTML at Wiley Online Library

Figures

Figure 2.4.1

Architecture of two common CCD sensors: the frame transfer sensor on the left and the interline transfer sensor on the right. The photosensitive elements are shown as various patterns and the charge transfer areas as solid gray.

View Image
Figure 2.4.2

Spectral sensitivity for CCDs in three different configurations, along with the performance of the human eye for both scotopic and photopic vision.

View Image
Figure 2.4.3

Pixel binning in a CCD sensor. In this example, the charge from four pixels is binned and sent to the output amplifier as a single voltage.

View Image
Figure 2.4.4

CCD photodiode array integrated circuit. A single chip color CCD sensor with an adherent color filter (Bayer filter).

View Image
Figure 2.4.5

Sequential color three-pass CCD imaging system. A single-sensor color CCD camera utilizing a rotating filter wheel to capture the red, green, and blue components of the color image as sequential frames.

View Image

Literature Cited

Literature Cited
	Inoué, S. and Spring, , K.R. 1997. Video Microscopy: The Fundamentals. Plenum, New York.
Key References
	Inoué and Spring 1997. See above.
A good general reference for microscope optics and electronic imaging.
	Shotton, D. 1993. Electronic Light Microscopy. Wiley-Liss, New York.
This text emphasizes image acquisition, sampling and image processing.
	Sluder, G. and Wolf, D.E. 1998. Methods in Cell Biology, Volume 56, Video Microscopy. Academic Press, San Diego.
Offers practical guidance in the acquisition of high quality electronic images of microscopic specimens.
Internet Resources
	http://microscopy.fsu.edu
A massive collection of static and dynamic information about optics, microscopy, image processing and related topics.