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Overview of Neural Gene Expression

Rachael L. Neve¹, Kim A. Neve²

¹Harvard Medical School and McLean Hospital, Belmont, Massachusetts
²Oregon Health Sciences University and Veterans Affairs Medical Center, Portland, Oregon

Publication Name:

Current Protocols in Neuroscience

Unit Number:

Unit 4.5

DOI:

10.1002/0471142301.ns0405s00

Online Posting Date:

May, 2001

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Abstract

This overview describes issues that must be considered before attempting to express neural cDNAs in mammalian cells, including the choice of expression vector and cell type. Considerations for introducing recombinant vectors into cells are discussed along with a comparison of achieving stable or transient expression. Finally, the appropriate promoter is crucial and must be chosen to fit the design of the expression system.

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Unit Introduction
Choice of Expression Vector
Choice of Cell Type
Introducing Recombinant Vectors into Cells
Transient or Stable Gene Expression
Choice of Promoter
Literature Cited
Tables

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Literature Cited

Literature Cited
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	Donis, J.A., Michelman, M.V., and Neve, R.L. 1993. Comparison of expression of a series of mammalian vector promoters in the neuronal cell lines PC12 and HT4. BioTechniques 15:786-787
	Fuerst, T.R., Niles, E.G., Studier, F.W., and Moss, B. 1986. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. U.S.A. 83: 8122- 8126.
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	Luckow, V.A. and Summers, M.D. 1988. Trends in the development of baculovirus expression vectors. Bio/Technology 6: 47- 55.
	Neve, R.L. and Geller, A.I. 1996. A defective herpes simplex virus vector system for gene delivery into the brain: Comparison with alternative gene delivery systems and usefulness for gene therapy. Clinical Neuroscience 3: 262- 267.
	No, D., Yao, T.P., and Evans, R.M. 1996. Ecdysone-inducible gene expression in mammalian cells and mice. Proc. Natl. Acad. Sci. U.S.A. 93: 3346- 3351.
	Rivera, V.M., Clackson, T., Nateson, S., Pollock, R., Amara, J.F., Keenen, T., Magari, S.R., Phillips, T., Courage, N.L., Cerasoli, F., Holt, D.A. Jr., and Gilman, M. 1996. A humanized system for pharmacologic control of gene expression Nature Med. 2: 1028- 1032.
	Shockett, P.E. and Schatz, D.G. 1996. Diverse strategies for tetracycline-regulated inducible gene expression. Proc. Natl. Acad. Sci. U.S.A. 93: 5173- 5176.
	Xia, Z., Dudek, H., Miranti, C.K., and Greenberg, M.E. 1996. Calcium influx via the NMDA receptor induces immediate early gene transcription by a MAP kinase/ERK–dependent mechanism. J.Neurosci. 16: 5425- 5436.