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Overview of Protein Expression in Saccharomyces cerevisiae

Robert L. Strausberg¹, Susan L. Strausberg²

¹National Institutes of Health, Bethesda, Maryland
²University of Maryland, Rockville, Maryland

Publication Name:

Current Protocols in Protein Science

Unit Number:

Unit 5.6

DOI:

10.1002/0471140864.ps0506s02

Online Posting Date:

May, 2001

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Abstract

This overview presents vectors and host strains that are available to direct gene expression in S. cerevisiae, including information on promoters, vector maintenance and copy number, transcription terminators, and selectable markers. Challenges to the expression of foreign proteins are also covered, including attainment of desired production yield, production of protein with appropriate post-translational modifications, conformation and function, and secretion to the extracellular medium.

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Unit Introduction
Vectors and Host Strains
Challenges to the Expression of Foreign Proteins
Time Frame and Future Prospects
Literature Cited
Figures
Tables

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Figures

Figure 5.6.1

Genetic constructions for expression of At-III. Abbreviations: GAL1, galactose; GDP1, glyceraldehyde-3-phosphate dehydrogenase; MF-1, -factor; PHO5, acid phosphatase; SUC2, invertase; TIS, transcription initiation site; UAS, upstream activation site.

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Literature Cited

Literature Cited
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