General Discussion

What is general transduction rate ( in a bacteria using any phage?)

I am doing trasductaion in Ecoli using p1 vir , using Ecoli gnome manipulation by p1 transducation.

when I plate the tranduced cell on Km- EGTA plates i hardly see 1 or 2 colinies. So I was wondering what would be the number on colonies we see is a good transuction?

Hello,

I am new to TEM sample preparation and I have been using "Current Protocols in Microbiology" as a guide.  There are three TEM primary fixative mentioned (1, 2 and 3) but no recipes.  Can someone provide me with the recipes?

Thanks!

Amy

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