Submitted by Esmeralda Meyer on July 20, 2010 - 1:10pm
I'm testing protocol 21.11.4 from Current Protocols in protein science. The denaturing buffer is said to contain 150 mM oxidized glutathione and 15 mM of reduced glutathione. In the past I've always used the reduced version 10X more than the oxidated. I would like to confirm those numbers. Thank you.
Submitted by Paul Wingfield on April 8, 2009 - 8:43am
Do the members have any recommendations for a commercial full protein sequencing service located in USA? I am specifically interested in a protein with two chains ~25,000 Mr each.
